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dc.contributor.authorDolcet Negre, Marta Maria
dc.contributor.authorTorres i Grifo, Mercè
dc.contributor.authorCanela i Garayoa, Ramon
dc.date.accessioned2016-10-14T11:29:57Z
dc.date.issued2016
dc.identifier.issn1871-6784
dc.identifier.urihttp://hdl.handle.net/10459.1/57931
dc.description.abstractThe use of mycelia as biocatalysts has technical and economic advantages. However, there are several difficulties in obtaining accurate results in mycelium-catalysed reactions. Firstly, sample extraction, indispensable because of the presence of mycelia, can bring into the extract components with a similar structure to that of the analyte of interest; secondly, mycelia can influence the recovery of the analyte. We prepared calibration standards of 3-phenoxy-1,2-propanediol (PPD) in the pure solvent and in the presence of mycelia (spiked before or after extraction) from five fungi (Aspergillus niger, Aspergillus tubingensis, Penicillium aurantiogriseum, Penicillium sp. and Aspergillus terreus). The quantification of PPD was carried out by HPLC–UV and UV–vis spectrophotometry. The manuscript shows that the last method is as accurate as the HPLC method. However, the colorimetric method led to a higher data throughput, which allowed the study of more samples in a shorter time. Matrix effects were evaluated visually from the plotted calibration data and statistically by simultaneously comparing the intercept and slope of calibration curves performed with solvent, post-extraction spiked standards and pre-extraction spiked standards. Significant differences were found between the post- and pre-extraction spiked matrix-matched functions. Pre-extraction spiked matrix-matched functions based on A. tubingensis mycelia, selected as the reference, were validated and used to compensate for low recoveries. These validated functions were successfully applied to the quantification of PPD achieved during the hydrolysis of glycidyl phenyl ether by mycelium-bound epoxide hydrolases and equivalent hydrolysis yields were determined by HPLC–UV and UV–vis spectrophotometry. This study may serve as starting point to implement matrix effects evaluation when mycelium-bound epoxide hydrolases are studied.ca_ES
dc.description.sponsorshipWe thank Ministry of Science and Innovation (MICINN) of Spain for the PhD fellowship of Marta Maria Dolcet (PPQ2003-02871).ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationMICYT/PN2000-2003/PPQ2003-02871ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.nbt.2016.02.003ca_ES
dc.relation.ispartofNew Biotechnology , 2016, vol. 33, núm. 4, p. 449-459
dc.rights(c) Elsevier, 2016ca_ES
dc.titleEffect of fungal mycelia on the HPLC–UV and UV–vis spectrophotometric assessment of mycelium-bound epoxide hydrolase using glycidyl phenyl etherca_ES
dc.typearticleca_ES
dc.identifier.idgrec024126
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.nbt.2016.02.003
dc.date.embargoEndDate10000-01-01


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