Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Tecnologia dels Aliments
  • Articles publicats (Tecnologia dels Aliments)
  • View Item
  •   Home
  • Recerca
  • Tecnologia dels Aliments
  • Articles publicats (Tecnologia dels Aliments)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Effect of fungal mycelia on the HPLC–UV and UV–vis spectrophotometric assessment of mycelium-bound epoxide hydrolase using glycidyl phenyl ether

Thumbnail
View/Open
024126.pdf (484.5Kb)
Sol·licita una còpia
Issue date
2016
Author
Dolcet Negre, Marta Maria
Torres i Grifo, Mercè
Canela i Garayoa, Ramon
Suggested citation
Dolcet Negre, Marta Maria; Torres i Grifo, Mercè; Canela i Garayoa, Ramon; . (2016) . Effect of fungal mycelia on the HPLC–UV and UV–vis spectrophotometric assessment of mycelium-bound epoxide hydrolase using glycidyl phenyl ether. New Biotechnology , 2016, vol. 33, núm. 4, p. 449-459. https://doi.org/10.1016/j.nbt.2016.02.003.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
The use of mycelia as biocatalysts has technical and economic advantages. However, there are several difficulties in obtaining accurate results in mycelium-catalysed reactions. Firstly, sample extraction, indispensable because of the presence of mycelia, can bring into the extract components with a similar structure to that of the analyte of interest; secondly, mycelia can influence the recovery of the analyte. We prepared calibration standards of 3-phenoxy-1,2-propanediol (PPD) in the pure solvent and in the presence of mycelia (spiked before or after extraction) from five fungi (Aspergillus niger, Aspergillus tubingensis, Penicillium aurantiogriseum, Penicillium sp. and Aspergillus terreus). The quantification of PPD was carried out by HPLC–UV and UV–vis spectrophotometry. The manuscript shows that the last method is as accurate as the HPLC method. However, the colorimetric method led to a higher data throughput, which allowed the study of more samples in a shorter time. Matrix effects were evaluated visually from the plotted calibration data and statistically by simultaneously comparing the intercept and slope of calibration curves performed with solvent, post-extraction spiked standards and pre-extraction spiked standards. Significant differences were found between the post- and pre-extraction spiked matrix-matched functions. Pre-extraction spiked matrix-matched functions based on A. tubingensis mycelia, selected as the reference, were validated and used to compensate for low recoveries. These validated functions were successfully applied to the quantification of PPD achieved during the hydrolysis of glycidyl phenyl ether by mycelium-bound epoxide hydrolases and equivalent hydrolysis yields were determined by HPLC–UV and UV–vis spectrophotometry. This study may serve as starting point to implement matrix effects evaluation when mycelium-bound epoxide hydrolases are studied.
URI
http://hdl.handle.net/10459.1/57931
DOI
https://doi.org/10.1016/j.nbt.2016.02.003
Is part of
New Biotechnology , 2016, vol. 33, núm. 4, p. 449-459
European research projects
Collections
  • Articles publicats (Tecnologia dels Aliments) [596]
  • Articles publicats (Química) [313]
  • Articles publicats (DBA Center) [38]
  • Publicacions de projectes de recerca del Plan Nacional [2956]

Contact Us | Send Feedback | Legal Notice
© 2023 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2023 BiD. Universitat de Lleida
Metadata subjected to