Genomic response programs of Candida albicans following protoplasting and regeneration

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2005-12-16
Author
Castillo, Luis
Martínez, Ana Isabel
Garcera, Ana
García-Martínez, José
Ruiz-Herrera, José
Valentín, Eulogio
Sentandreu, Rafael
Suggested citation
Castillo, Luis; Martínez, Ana Isabel; Garcera, Ana; García-Martínez, José; Ruiz-Herrera, José; Valentín, Eulogio; Sentandreu, Rafael; . (2005) . Genomic response programs of Candida albicans following protoplasting and regeneration. Fungal Genetics and Biology, 2005, num. 43, p. 124-134. https://doi.org/10.1016/j.fgb.2005.12.002.
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Abstract
Transcription proWling of Candida albicans cells responding to the elimination of the wall (protoplasts) and posterior regeneration was explored. DNA microarrays were used to measure changes in the expression of 6039 genes, and the upregulated genes during regeneration at 28 °C were assigned to fourteen categories. A total of 407 genes were upregulated during the process, of which 144 reached a maximum after 1 h. MKC1, a gene encoding a member of the regulatory pathway involved in cell wall integrity was overexpressed. Time-dependent expression divided the genes into 40 clusters. Clusters 1-19 were highly expressed initially (time 0) and downregulated following incubation, whereas transcription of the genes grouped into clusters 20-40 showed the opposite behaviour. These results suggest that the Wrst clusters group genes permitting the cell adaptation to a sub-optimal environment due to removal of the wall, whereas the second group represents genes required for protoplasts regeneration after shifted to optimal conditions from 4 to 28 °C.
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http://hdl.handle.net/10459.1/57914
DOI
https://doi.org/10.1016/j.fgb.2005.12.002
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Fungal Genetics and Biology, 2005, num. 43, p. 124-134
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