Transcriptional regulation of the rice arginine decarboxylase (Adc1) and S-adenosylmethionine decarboxylase (Samdc) genes by methyl jasmonate
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We investigated the effect of methyl jasmonate (MeJa) treatment on the expression of two genes in the rice polyamine biosynthesis pathway and on the polyamine content in wild type plants and transgenic rice plants expressing a Datura stramonium (Ds) Adc cDNA, the latter accumulating up to three-fold the normal level of putrescine. Exogenous MeJa transiently inhibited the expression of OsAdc1, OsSamdc and Spermidine synthase (OsSpds) genes in the polyamine biosynthesis pathway, probably through transcriptional repression. There was also a similar negative impact on the DsAdc transgene in transgenic plants, even though a constitutive promoter was used to drive transgene expression. The free putrescine content was reduced significantly in the leaves of both wild type and transgenic plants in response to MeJa, although the magnitude of the effect was greater in wild type plants. We discuss our findings with respect to the previously proposed threshold model of polyamine metabolism in plants subjected to abiotic stress.
Is part ofPlant Physiology and Biochemistry, 2010, vol. 48, núm. 7, p. 553–559
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Peremartí, Ariadna; Bassie Rene, Ludovic; Zhu, Changfu; Christou, Paul; Capell Capell, Teresa (Springer Verlag, 2010)Arginine decarboxylase (ADC) is a key enzyme in plants that converts arginine into putrescine, an important mediator of abiotic stress tolerance. Adc genes have been isolated from a number of dicotyledonous plants but ...
Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase Peremartí, Ariadna; Bassie Rene, Ludovic; Christou, Paul; Capell Capell, Teresa (Springer Verlag, 2009)Polyamines are known to play important roles in plant stress tolerance but it has been difficult to determine precise functions for each type of polyamine and their interrelationships. To dissect the roles of putrescine ...
Transgenic wheat plants expressing an oat arginine decarboxylase cDNA exhibit increases in polyamine content in vegetative tissue and seeds Bassie Rene, Ludovic; Zhu, Changfu; Capell Capell, Teresa; Romagosa Clariana, Ignacio; Christou, Paul (Springer, 2008)We generated and characterized a trans- genic wheat ( Triticum aestivum L.) population expressing constitutively an oat arginine decarboxylase ( ADC ) cDNA. Transgenic wheat plants expressing ...