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dc.contributor.authorPérez Sampietro, María
dc.contributor.authorSerra Cardona, Albert
dc.contributor.authorCanadell, David
dc.contributor.authorCasas Herranz, Celia
dc.contributor.authorAriño Carmona, Joaquín
dc.contributor.authorHerrero Perpiñán, Enrique
dc.description.abstractThe yeast Saccharomyces cerevisiae is employed as a model to study the cellular mechanisms of toxicity and defense against selenite, the most frequent environmental selenium form. We show that yeast cells lacking Aft2, a transcription factor that together with Aft1 regulates iron homeostasis, are highly sensitive to selenite but, in contrast to aft1 mutants, this is not rescued by iron supplementation. The absence of Aft2 strongly potentiates the transcriptional responses to selenite, particularly for DNA damage- and oxidative stress-responsive genes, and results in intracellular hyperaccumulation of selenium. Overexpression of PHO4, the transcriptional activator of the PHO regulon under low phosphate conditions, partially reverses sensitivity and hyperaccumulation of selenite in a way that requires the presence of Spl2, a Pho4-controlled protein responsible for post-transcriptional downregulation of the low-affinity phosphate transporters Pho87 and Pho90. SPL2 expression is strongly downregulated in aft2 cells, especially upon selenite treatment. Selenite hypersensitivity of aft2 cells is fully rescued by deletion of PHO90, suggesting a major role for Pho90 in selenite uptake. We propose that the absence of Aft2 leads to enhanced Pho90 function, involving both Spl2-dependent and independent events and resulting in selenite hyperaccumulation and toxicity.ca_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (grant BFU2010-17656), the Generalitat de Catalunya (grant 2009/SGR/196) and the University of Lleida to E.H., and by the Spanish Ministry of Science and Innovation and the Fondo Europeo de Desarrollo Regional (grants BFU2011-30197-C3-01 and BFU2014-54591-C2-1-P), and the Generalitat de Catalunya (grant 2014SGR-4) to J.A.ca_ES
dc.publisherScientific Reportsca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofScientific Reports, 2016, vol. 6, núm. 32836ca_ES
dc.rightscc-by (c) Pérez Sampietro et al., 2016ca_ES
dc.titleThe yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport systemca_ES

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