The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system

View/ Open
Issue date
2016Author
Pérez Sampietro, María
Serra Cardona, Albert
Canadell, David
Ariño Carmona, Joaquín
Suggested citation
Pérez Sampietro, María;
Serra Cardona, Albert;
Canadell, David;
Casas Herranz, Celia;
Ariño Carmona, Joaquín;
Herrero Perpiñán, Enrique;
.
(2016)
.
The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system.
Scientific Reports, 2016, vol. 6, núm. 32836.
https://doi.org/10.1038/srep32836.
Metadata
Show full item recordAbstract
The yeast Saccharomyces cerevisiae is employed as a model to study the cellular mechanisms of toxicity and defense against selenite, the most frequent environmental selenium form. We show that yeast cells lacking Aft2, a transcription factor that together with Aft1 regulates iron homeostasis, are highly sensitive to selenite but, in contrast to aft1 mutants, this is not rescued by iron supplementation. The absence of Aft2 strongly potentiates the transcriptional responses to selenite, particularly for DNA damage- and oxidative stress-responsive genes, and results in intracellular hyperaccumulation of selenium. Overexpression of PHO4, the transcriptional activator of the PHO regulon under low phosphate conditions, partially reverses sensitivity and hyperaccumulation of selenite in a way that requires the presence of Spl2, a Pho4-controlled protein responsible for post-transcriptional downregulation of the low-affinity phosphate transporters Pho87 and Pho90. SPL2 expression is strongly downregulated in aft2 cells, especially upon selenite treatment. Selenite hypersensitivity of aft2 cells is fully rescued by deletion of PHO90, suggesting a major role for Pho90 in selenite uptake. We propose that the absence of Aft2 leads to enhanced Pho90 function, involving both Spl2-dependent and independent events and resulting in selenite hyperaccumulation and toxicity.
Is part of
Scientific Reports, 2016, vol. 6, núm. 32836European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Pérez Sampietro et al., 2016
Related items
Showing items related by title, author, creator and subject.
-
Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: a quantitative study
Petrezsélyová, Silvia; López Malo, María; Canadell, David; Roque, Alicia; Serra Cardona, Albert; Marqués, M. Carmen; Vilaprinyo Terré, Ester; Alves, Rui; Yenush, Lynne; Ariño Carmona, Joaquín (Public Library of Science, 2016)Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling ... -
Comprehensive transcriptional analysis of the oxidative response in yeast
Molina Navarro, Maria Micaela; Castells i Roca, Laia; Bellí i Martínez, Gemma; García-Martínez, José; Marín-Navarro, Julia; Moreno, Joaquín; Pérez-Ortín, José E.; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2008)The oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm has been adapted for a dynamic ... -
Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria
Molina Navarro, Maria Micaela; Casas Herranz, Celia; Piedrafita Llorens, Lídia; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique (Elsevier, 2003)The Saccharomyces cerevisiae monothiol glutare- doxin Grx5 participates in the mitochondrial biogenesis of iron–sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans. Chicken (cGRX5) and human ...