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dc.contributor.authorCrespo Sempere, Ana
dc.contributor.authorEstiarte, Núria
dc.contributor.authorMarín Sillué, Sònia
dc.contributor.authorSanchís Almenar, Vicente
dc.contributor.authorRamos Girona, Antonio J.
dc.date.accessioned2016-08-31T08:08:31Z
dc.date.available2016-08-31T08:08:31Z
dc.date.issued2015-01-31
dc.identifier.issn0740-0020
dc.identifier.urihttp://hdl.handle.net/10459.1/57759
dc.description.abstractFusarium graminearum not only reduces yield and seed quality but also constitutes a risk to public or animal health owing to its ability to contaminate grains with mycotoxins. Resistance problems are emerging and control strategies based on new targets are needed. Polyamines have a key role in growth, development and differentiation. In this work, the possibility of using polyamine metabolism as a target to control F. graminearum has been assessed. It was found that putrescine induces mycotoxin production, correlating with an over expression of TRI5 and TRI6 genes. In addition, a homolog of the Saccharomyces cerevisiae TPO4 involved in putrescine excretion was up-regulated as putrescine concentration increased while DUR3 and SAM3 homologues, involved in putrescine uptake, were down-regulated. When 2.5 mM D,L-alpha-difluoromethylornithine (DFMO) was added to the medium, DON production decreased from 3.2 to 0.06 ng/mm2 of colony and growth was lowered by up to 70 per cent. However, exogenous putrescine could overcome DFMO effects. Five polyamine transport inhibitors were also tested against F. graminearum. AMXT-1505 was able to completely inhibit in vitro growth and DON production. Additionally, AMXT-1505 blocked F. graminearum growth in inoculated wheat spikes reducing DON mycotoxin contamination from 76.87 µg/g to 0.62 µg/g.
dc.description.sponsorshipThis research has been supported by the Spanish Government (AGL2011-24862 project). N. Estiarte thanks the Comissionat per a Universitats i Recerca, del Departament d'Innovació, Universitats i Empresa de la Generalitat de Catalunya (AGAUR), for her predoctoral grant (FI-DGR 2013).
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relationMICINN/PN2008-2011/AGL2011-24862
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.fm.2015.01.020
dc.relation.ispartofFood Microbiology, 2015, vol. 49, p. 95-103
dc.rightscc-by-nc-nd (c) Elsevier, 2015
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/deed
dc.subjectPoliaminas
dc.subjectDeoxinivalenol
dc.subjectTrigo
dc.subject.classificationBlat
dc.subject.classificationPoliamines
dc.subject.classificationTecnologia dels aliments
dc.subject.otherWheat
dc.subject.otherPolyamines
dc.subject.otherFood technology
dc.titleTargeting Fusarium graminearum control via polyamine enzyme inhibitors and polyamine analogs
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2016-08-03T08:22:56Z
dc.identifier.idgrec022368
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.fm.2015.01.020


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cc-by-nc-nd (c) Elsevier, 2015
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2015