Targeting Fusarium graminearum control via polyamine enzyme inhibitors and polyamine analogs
Data de publicació2015-01-31
Crespo Sempere, Ana
MetadadesMostra el registre d'unitat complet
Fusarium graminearum not only reduces yield and seed quality but also constitutes a risk to public or animal health owing to its ability to contaminate grains with mycotoxins. Resistance problems are emerging and control strategies based on new targets are needed. Polyamines have a key role in growth, development and differentiation. In this work, the possibility of using polyamine metabolism as a target to control F. graminearum has been assessed. It was found that putrescine induces mycotoxin production, correlating with an over expression of TRI5 and TRI6 genes. In addition, a homolog of the Saccharomyces cerevisiae TPO4 involved in putrescine excretion was up-regulated as putrescine concentration increased while DUR3 and SAM3 homologues, involved in putrescine uptake, were down-regulated. When 2.5 mM D,L-alpha-difluoromethylornithine (DFMO) was added to the medium, DON production decreased from 3.2 to 0.06 ng/mm2 of colony and growth was lowered by up to 70 per cent. However, exogenous putrescine could overcome DFMO effects. Five polyamine transport inhibitors were also tested against F. graminearum. AMXT-1505 was able to completely inhibit in vitro growth and DON production. Additionally, AMXT-1505 blocked F. graminearum growth in inoculated wheat spikes reducing DON mycotoxin contamination from 76.87 µg/g to 0.62 µg/g.
És part deFood Microbiology, 2015, vol. 49, p. 95-103
Projectes de recerca europeus
Mostrant elements relacionats per títol, autor i matèria.
Exploring polyamine metabolism of Alternaria alternata to target new substances to control the fungal infection. Estiarte, Núria; Crespo Sempere, Ana; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier Ltd., 2017-02-03)Polyamines are essential for all living organisms as they are involved in several vital cell functions. The biosynthetic pathway of polyamines and its regulation is well established and, in this sense, the ornithine ...
Propidium monoazide combined with real-time quantitative PCR to quantify viable Alternaria spp. contamination in tomato products Crespo Sempere, Ana; Estiarte, Núria; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier, 2013)Alternaria is a common contaminating genus of fungi in fruits, grains, and vegetables that causes severe economic losses to farmers and the food industry. Furthermore, it is claimed that Alternaria spp. are able to produce ...
Effect of 1-methylcyclopropene on the development of black mold disease and its potential effect on alternariol and alternariol monomethyl ether biosynthesis on tomatoes infected with Alternaria alternata Estiarte, Núria; Crespo Sempere, Ana; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier, 2016)Ethylene is a naturally produced plant regulator involved in several plant functions, such as regulation of fruit ripening. Inhibition of ethylene perception by using 1-methylcyclopropene (1-MCP) slows down the ripening ...