Browsing Articles publicats (Agrotecnio Center) by Author "Bai, Chao"
Now showing items 1-7 of 7
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A novel carotenoid, 4-keto-a-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus
Breitenbach, Jürgen; Bai, Chao; Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu; Sandmann, Gerhard (Elsevier, 2014)Rice endosperm is devoid of carotenoids because the initial biosynthetic steps are absent. The early carotenogenesis reactions were constituted through co-transformation of endosperm-derived rice callus with phytoene ... -
An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation
Bai, Chao; Rivera Vélez, Sol Maiam; Medina Piles, Vicente; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2014)We have developed an assay based on rice embryogenic callus for rapid functional characterization of metabolic genes. We validated the assay using a selection of well-characterized genes with known functions in the carotenoid ... -
Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor–product balance
Bai, Chao; Capell Capell, Teresa; Berman Quintana, Judit; Medina Piles, Vicente; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2016)The profile of secondary metabolites in plants reflects the balance of biosynthesis, degradation and storage, including the availability of precursors and products that affect the metabolic equilibrium. We investigated the ... -
Cloning and functional analysis of the promoters that upregulate carotenogenic gene expression during flower development in Gentiana lutea
Zhu, Changfu; Yang, Qingjie; Ni, Xiuzhen; Bai, Chao; Sheng, Yanmin; Shi, Lianxuan; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul (Wiley, 2014)Over the last two decades, many carotenogenic genes have been cloned and used to generate metabolically engineered plants producing higher levels of carotenoids. However, comparatively little is known about the regulation ... -
Fast quantitative method for the analysis of carotenoids in transgenic maize
Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon (American Chemical Society, 2013)A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass ... -
The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints
Pérez Massot, Eduard; Banakar, Raviraj; Gómez Galera, Sonia; Zorrilla López, Uxue; Sanahuja Solsona, Georgina; Arjó Pont, Gemma; Miralpeix i Anglada, Bruna; Vamvaka, Evangelia; Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Dashevskaya, Svetlana; Berman Quintana, Judit; Sabalza Gallués, Maite; Yuan, Dawei; Bai, Chao; Bassie Rene, Ludovic; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Springer Verlag, 2013)Malnutrition is a prevalent and entrenched global socioeconomic challenge that reflects the combined impact of poverty, poor access to food, inefficient food distribution infrastructure, and an over-reliance on ... -
ZmPBF and ZmGAMYB transcription factors independently transactivate the promoter of the maize (Zea mays L.) β-carotene hydroxylase 2 gene
Jin, Xin; Bai, Chao; Bassie Rene, Ludovic; Nogareda, Carmina; Romagosa Clariana, Ignacio; Twyman, Richard M.; Christou, Paul; Zhu, Changfu (Wiley, 2018-12-01)The maize (Zea mays) enzyme b-carotene hydroxylase 2 (ZmBCH2) controls key steps in the conversion of b-carotene to zeaxanthin in the endosperm. The ZmBCH2 gene has an endosperm-preferred and developmentally regulated ...