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dc.contributor.authorPetrezsélyová, Silvia
dc.contributor.authorLópez Malo, María
dc.contributor.authorCanadell, David
dc.contributor.authorRoque, Alicia
dc.contributor.authorSerra Cardona, Albert
dc.contributor.authorMarqués, M. Carmen
dc.contributor.authorVilaprinyo Terré, Ester
dc.contributor.authorAlves, Rui
dc.contributor.authorYenush, Lynne
dc.contributor.authorAriño Carmona, Joaquín
dc.description.abstractRegulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calciumregulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response. Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1- binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%. The models suggest the existence of a second input with similar kinetics, which would be likely mediated by high pH-induced activation of the Snf1 kinase.ca_ES
dc.description.sponsorshipThis work was supported by grants BFU2011-30197-C3-01, BFU2014-54591-C2-1-P and EUI2009-04147 (SysMo2) to JA. (Ministry of Industry and Competitivity, Spain, and Fondo Europeo de Desarrollo Regional [FEDER]). JA is the recipient of an Ajut 2014SGR-4 award (Generalitat de Catalunya). DC was recipient of a predoctoral fellowship from the Spanish Ministry of Education.ca_ES
dc.publisherPublic Library of Scienceca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofPlos One, 2016, vol. 11, núm. 6, e0158424ca_ES
dc.rightscc-by (c) Petrezsélyová et al., 2016ca_ES
dc.titleRegulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: a quantitative studyca_ES

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cc-by (c) Petrezsélyová et al., 2016
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