Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: a quantitative study

View/ Open
Issue date
2016Author
Petrezsélyová, Silvia
López Malo, María
Canadell, David
Roque, Alicia
Serra Cardona, Albert
Marqués, M. Carmen
Yenush, Lynne
Ariño Carmona, Joaquín
Suggested citation
Petrezsélyová, Silvia;
López Malo, María;
Canadell, David;
Roque, Alicia;
Serra Cardona, Albert;
Marqués, M. Carmen;
...
Ariño Carmona, Joaquín.
(2016)
.
Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: a quantitative study.
Plos One, 2016, vol. 11, núm. 6, e0158424.
https://doi.org/10.1371/journal.pone.0158424.
Metadata
Show full item recordAbstract
Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt
and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression
is under the control of diverse signaling pathways, including that mediated by the calciumregulatable
protein phosphatase calcineurin and its downstream transcription factor Crz1.
We present here a quantitative study of the expression of Ena1 in response to alkalinization
of the environment and we analyze the contribution of Crz1 to this response. Experimental
data and mathematical models substantiate the existence of two stress-responsive Crz1-
binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early
response of the ENA1 promoter is about 60%. The models suggest the existence of a second
input with similar kinetics, which would be likely mediated by high pH-induced activation
of the Snf1 kinase.
Is part of
Plos One, 2016, vol. 11, núm. 6, e0158424European research projects
Collections
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Petrezsélyová et al., 2016
Related items
Showing items related by title, author, creator and subject.
-
The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system
Pérez Sampietro, María; Serra Cardona, Albert; Canadell, David; Casas Herranz, Celia; Ariño Carmona, Joaquín; Herrero Perpiñán, Enrique (Scientific Reports, 2016)The yeast Saccharomyces cerevisiae is employed as a model to study the cellular mechanisms of toxicity and defense against selenite, the most frequent environmental selenium form. We show that yeast cells lacking Aft2, a ... -
Use of physiological constraints to identify quantitative design principles for gene expression in yeast adaptation to heat shock
Vilaprinyo Terré, Ester; Alves, Rui; Sorribas Tello, Albert (BioMed Central, 2006)Background: Understanding the relationship between gene expression changes, enzyme activity shifts, and the corresponding physiological adaptive response of organisms to environmental cues is crucial in explaining how cells ... -
Quantitative operating principles of yeast metabolism during adaptation to heat stress
Pereira, Tania; Vilaprinyo Terré, Ester; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique; Salvadó López, Baldiri; Sorribas Tello, Albert; Altés, Gisela; Alves, Rui (Elsevier, 2018)Microorganisms evolved adaptive responses to survive stressful challenges in ever-changing environments. Understanding the relationships between the physiological/metabolic adjustments allowing cellular stress adaptation ...