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dc.contributor.authorPalatsi Civit, Jordi
dc.contributor.authorIlla i Alibés, Josep
dc.contributor.authorPrenafeta-Boldú, Francesc Xavier
dc.contributor.authorLaureni, M.
dc.contributor.authorFernández García, Belén
dc.contributor.authorAngelidaki, I.
dc.contributor.authorFlotats i Ripoll, Xavier
dc.date.accessioned2016-06-21T08:37:04Z
dc.date.issued2010
dc.identifier.issn0960-8524
dc.identifier.urihttp://hdl.handle.net/10459.1/57242
dc.description.abstractBiomass samples taken during the continuous operation of thermophilic anaerobic digestors fed with manure and exposed to successive inhibitory pulses of long-chain fatty acids (LCFA) were characterized in terms of specific metabolic activities and 16S rDNA DGGE profiling of the microbial community structure. Improvement of hydrogenotrophic and acidogenic (b-oxidation) activity rates was detected upon successive LCFA pulses, while different inhibition effects over specific anaerobic trophic groups were observed. Bioreactor recovery capacity and biomass adaptation to LCFA inhibition were verified. Population profiles of eubacterial and archaeal 16S rDNA genes revealed that no significant shift on microbial community composition took place upon biomass exposure to LCFA. DNA sequencing of predominant DGGE bands showed close phylogenetic affinity to ribotypes characteristic from specific b-oxidation bacterial genera (Syntrophomonas and Clostridium), while a single predominant syntrophic archaeae was related with the genus Methanosarcina. The hypothesis that biomass adaptation was fundamentally of physiological nature was tested using mathematical modelling, taking the IWA ADM1 as general model. New kinetics considering the relation between LCFA inhibitory substrate concentration and specific biomass content, as an approximation to the adsorption process, improved the model fitting and provided a better insight on the physical nature of the LCFA inhibition process.ca_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (Projects ENE 2004-00724 and ENE 2007-65850) and from the Danish Energy Council (EFP-05 Journal no.: 33031-0029).ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationMIECI/PN2004-2007/ENE2004-00724/ALTca_ES
dc.relationMIECI/PN2004-2007/ENE2007-65850
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.biortech.2009.11.069ca_ES
dc.relation.ispartofBioresource Technology, 2010, vol. 101, núm. 7, p. 2243-2251ca_ES
dc.rights(c) Elsevier, 2010ca_ES
dc.subjectThermophilic anaerobic digestionca_ES
dc.subjectLCFA inhibition–adaptationca_ES
dc.subject16S rDNA profilingca_ES
dc.subjectADM1 modelca_ES
dc.titleLong-chain fatty acids inhibition and adaptation process in anaerobic thermophilic digestion: Batch tests, microbial community structure and mathematical modellingca_ES
dc.typearticleca_ES
dc.identifier.idgrec018626
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.biortech.2009.11.069
dc.date.embargoEndDate10000-01-01


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