Sustained activation of renal N-methyl-D-aspartate receptors decreases vitamin D synthesis: a possible role for glutamate on the onset of secondary HPT
Parisi Capdevila, Eva
Bozić Stanojević, Milica
Panizo García, Sara
MetadataShow full item record
N-methyl-D-aspartate (NMDA) receptors (NMDAR) are tetrameric amino acid receptors that act as membrane calcium channels. The presence of the receptor has been detected in the principal organs responsible for calcium homeostasis (kidney, bone, and parathyroid gland), pointing to a possible role in mineral metabolism. The aim of this study was to test the effect of NMDAR activation in the kidney and on 1,25(OH)2D3 synthesis. We determined the presence of NMDAR subunits in HK-2 (human kidney cells) cells and proved its functionality. NMDA treatment for 4 days induced a decrease in 1 -hydroxylase levels and 1,25(OH)2D3 synthesis through the activation of the MAPK/ERK pathway in HK-2 cells. In vivo administration of NMDA for 4 days also caused a decrease in blood 1,25(OH)2D3 levels in healthy animals and an increase in blood PTH levels. This increase in PTH induced a decrease in the urinary excretion of calcium and an increase in urinary excretion of phosphorous and sodium as well as in diuresis. Bone turnover markers also increased. Animals with 5/6 nephrectomy showed low levels of renal 1 -hydroxylase as well as high levels of renal glutamate compared with healthy animals. In conclusion, NMDAR activation in the kidney causes a decrease in 1,25(OH)2D3 synthesis, which induces an increase on PTH synthesis and release. In animals with chronic kidney disease, high renal levels of glutamate could be involved in the downregulation of 1 -hydroxylase expression.
Is part ofAJP- Endocrinology and Metabolism, 2010, vol. 299, núm. 5, p. E825-E831
European research projects
Showing items related by title, author, creator and subject.
Parisi Capdevila, Eva; Almadén, Yolanda; Ibarz Escuer, Mercedes; Panizo García, Sara; Cardús i Figueras, Anna; Rodríguez, Mariano; Fernández i Giráldez, Elvira; Valdivielso Revilla, José Manuel (American Physiological Society, 2009)N-methyl-d-aspartate receptors (NMDAR) are tetrameric amino acid receptors which act as membrane calcium channels. The presence of the receptor has been detected in the principal organs responsible for calcium homeostasis ...
Parisi Capdevila, Eva; Reñé Espinet, Josep Maria; Cardús i Figueras, Anna; Valcheva, Petya; Piñol Felis, Carme; Valdivielso Revilla, José Manuel; Fernández i Giráldez, Elvira (Elsevier, 2008)A high expression of vitamin D receptor (VDR) in colorectal cancer (CRC) tumoral tissue has been related to a good prognosis and it has been proposed that it could be a good biological marker of CRC progression. Nevertheless, ...
Hepatocyte vitamin D receptor regulates lipid metabolism and mediates experimental diet-induced steatosis Bozić Stanojević, Milica; Guzmán, Carla; Benet, Marta; Sánchez-Campos, Sonia; García-Monzón, Carmelo; Garí Marsol, Eloi; Gatius Calderó, Sònia; Valdivielso Revilla, José Manuel; Jover, Ramiro (Elsevier, 2016)Background & Aims The pathogenesis and progression of non-alcoholic fatty liver disease (NAFLD) is still incompletely understood. Several nuclear receptors play a role in liver lipid metabolism and can promote ...