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dc.contributor.authorGómez Pastor, Rocío
dc.contributor.authorPérez Torrado, Roberto
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorMatallana, Emilia
dc.date.accessioned2016-06-08T08:00:56Z
dc.date.issued2010
dc.identifier.issn1567-1356
dc.identifier.urihttp://hdl.handle.net/10459.1/57173
dc.description.abstractTranscriptome and proteome profiles have been established for the commercial wine yeast strain T73 during an important industrial process: yeast biomass propagation. The data from both analyses reveal that the metabolic transition from fermentation to respiration is the most critical step in biomass propagation. We identified 177 ORFs and 56 proteins among those most expressed during the process, thus highlighting cell stress response, mitochondrial and carbohydrate metabolism as the most represented functional categories. A direct correlation between mRNA changes and protein abundance was observed for several functional categories such as tricarboxylic acid cycle proteins, heat shock proteins, chaperons and oxidative stress response-related proteins. However, we found no concordance in the transcript and proteomic levels for glycolytic proteins, which is probably due to post-translational modifications increasing the number of protein isoforms, especially at the end of biomass propagation. The correlation between protein abundance and the enzyme activities of alcohol dehydrogenase, pyruvate decarboxylase and glyceraldehyde-3-phosphate dehydrogenase was not affected by these modifications. We suggest post-translational mechanisms during biomass propagation that affect the stability of those proteins that play an important role in the produced biomass' fermentative capacity.ca_ES
dc.description.sponsorshipThis work has been supported by grants AGL 2005-00508 and AGL 2008-00060 from the Spanish Ministry of Education and Science (MEC), Spain. R.G.-P. is a predoctoral fellow of the I3P program of ‘Consejo Superior de Investigaciones Científicas’ (Spain).ca_ES
dc.language.isoengca_ES
dc.publisherOxford University Pressca_ES
dc.relationMIECI/PN2004-2007/AGL2005-00508
dc.relationMICINN/PN2008-2011/AGL2008-00060
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1111/j.1567-1364.2010.00667.xca_ES
dc.relation.ispartofFEMS Yeast Research, 2010, vol.10, p. 870-884ca_ES
dc.rights(c) Oxford University Press, 2010ca_ES
dc.subjectBiomass propagationca_ES
dc.subjectWine yeastca_ES
dc.subjectProteomeca_ES
dc.titleTranscriptomic and proteomic insights of the wine yeast biomass propagation processca_ES
dc.typearticleca_ES
dc.identifier.idgrec015486
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/j.1567-1364.2010.00667.x
dc.date.embargoEndDate10000-01-01


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