N-methyl-D-aspartate receptors are expressed in rat parathyroid gland and regulate PTH secretion
Parisi Capdevila, Eva
Panizo García, Sara
Cardús i Figueras, Anna
MetadataShow full item record
N-methyl-d-aspartate receptors (NMDAR) are tetrameric amino acid receptors which act as membrane calcium channels. The presence of the receptor has been detected in the principal organs responsible for calcium homeostasis (kidney and bone), pointing to a possible role in mineral metabolism. In the present work, the presence of the receptor was determined in normal parathyroid glands (PTG) by real-time PCR, immunoprecipitation, and immunohistrochemistry. Healthy animals showed a decrease in blood parathyroid hormone (PTH) levels 15 min after the treatment with NMDA. This effect was also observed in animals with high levels of PTH-induced EDTA injection, but not in uremic animals with secondary hyperparathyroidism (2HPT). Normal rat PTG incubated in media with low calcium concentration (0.8 mM CaCl2) showed a decrease in PTH release when NMDA was added to the media. This effect of NMDA was abolished when glands were coincubated with MK801 (a pharmacological blocker of the NMDA channel) or PD98059 (an inhibitor of the ERK-MAPK pathway). Glands obtained from animals with 2HPT showed no effect of NMDA in the in vitro release of PTH, together with a decrease in the expression of NMDAR1. In conclusion, NMDA receptor is present in PTG and is involved in the regulation of the PTH release. The mechanism by which NMDAR exerts its function is through the activation of the MAPK cascade. In uremic 2HPT animals the receptor expression is downregulated and the treatment with NMDA does not affect PTH secretion.
Is part ofAJP - Renal Physiology, 2009, vol. 296, núm. 6, p. 1291-1296
European research projects
Showing items related by title, author, creator and subject.
Sustained activation of renal N-methyl-D-aspartate receptors decreases vitamin D synthesis: a possible role for glutamate on the onset of secondary HPT Parisi Capdevila, Eva; Bozić Stanojević, Milica; Ibarz Escuer, Mercedes; Panizo García, Sara; Valcheva, Petya; Coll, Blai; Fernández i Giráldez, Elvira; Valdivielso Revilla, José Manuel (American Physiological Society, 2010)N-methyl-D-aspartate (NMDA) receptors (NMDAR) are tetrameric amino acid receptors that act as membrane calcium channels. The presence of the receptor has been detected in the principal organs responsible for ...
Parisi Capdevila, Eva; Reñé Espinet, Josep Maria; Cardús i Figueras, Anna; Valcheva, Petya; Piñol Felis, Carme; Valdivielso Revilla, José Manuel; Fernández i Giráldez, Elvira (Elsevier, 2008)A high expression of vitamin D receptor (VDR) in colorectal cancer (CRC) tumoral tissue has been related to a good prognosis and it has been proposed that it could be a good biological marker of CRC progression. Nevertheless, ...
1,25-Dihydroxyvitamin D3 regulates VEGF production through a vitamin D response element in the VEGF promoter Cardús i Figueras, Anna; Panizo García, Sara; Encinas Martín, Mario; Dolcet Roca, Xavier; Gallego, Carme; Aldea, Martí; Fernandez, Elvira; Valdivielso Revilla, José Manuel (Elsevier, 2009)In previous studies we have demonstrated that the active form of vitamin D (1,25(OH)2D3) increases vascular endothelial growth factor (VEGF) expression and release in vascular smooth muscle cells (VSMC) in vitro. However, ...