Protein oxidation in Huntington disease affects energy production and vitamin B6 metabolism

Sorolla Bardají, Maria Alba; Rodríguez Colman, Maria José; Tamarit Sumalla, Jordi; Ortega, Zaira; Lucas, José J.; Ferrer, Isidre; Ros Salvador, Joaquim; Cabiscol Català, Elisa

doi:https://doi.org/10.1016/j.freeradbiomed.2010.05.016

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Issue date

2010

Author

Sorolla Bardají, Maria Alba

Rodríguez Colman, Maria José

Tamarit Sumalla, Jordi

Ortega, Zaira

Lucas, José J.

Ferrer, Isidre

Ros Salvador, Joaquim

Cabiscol Català, Elisa

Suggested citation

Sorolla Bardají, Maria Alba; Rodríguez Colman, Maria José; Tamarit Sumalla, Jordi; Ortega, Zaira; Lucas, José J.; Ferrer, Isidre; ... Cabiscol Català, Elisa. (2010) . Protein oxidation in Huntington disease affects energy production and vitamin B6 metabolism. Free Radical Biology and Medicine, 2010, vol. 49, núm. 4, p. 612-621. https://doi.org/10.1016/j.freeradbiomed.2010.05.016.

Abstract

Huntington disease (HD) is an inherited neurodegenerative disorder that initially affects the striatum and progressively the cortex. Oxidative stress in HD has been described as important to disease progression. In this study, protein carbonylation, used as a marker of protein oxidation, was analyzed

in human brain striatum. A comparison of HD samples tomatched controls identified 13 carbonylated proteins, including enzymes involved in the glycolytic pathway and mitochondrial proteins related to ATP production. Oxidation of the mitochondrial enzymes resulted in decreased catalytic activity, in good agreement with the energy deficiency observed in HD. Wealso found carbonylation of pyridoxal kinase and antiquitin 1, both involved in themetabolismof pyridoxal 5- phosphate, the active formof vitamin B6. The Tet/HD94 conditionalmousemodel allowed us to demonstrate that increased carbonylation in striatum is dependent on mutant huntingtin expression. As in humans, pyridoxal kinase showed decreased levels and was highly carbonylated in the gene-on mice; these modifications were reverted in the gene-offmice. Wehypothesize that both pyridoxal kinase and antiquitin 1 oxidation could result in decreased pyridoxal 5-phosphate availability necessary as a cofactor in transaminations, synthesis of glutathione, and synthesis of GABA and dopamine, two neurotransmitters that play a key role in HD pathology.

URI

http://hdl.handle.net/10459.1/57169

DOI

https://doi.org/10.1016/j.freeradbiomed.2010.05.016

Is part of

Free Radical Biology and Medicine, 2010, vol. 49, núm. 4, p. 612-621