Ascorbic acid synthesis and metabolism in maize are subject to complex and genotype-dependent feedback regulation duringendosperm development

Sanahuja Solsona, Georgina; Farré Martinez, Gemma; Bassie Rene, Ludovic; Zhu, Changfu; Christou, Paul; Capell Capell, Teresa

doi:https://doi.org/10.1002/biot.201300064

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Issue date

2013

Author

Sanahuja Solsona, Georgina

Farré Martinez, Gemma

Bassie Rene, Ludovic

Zhu, Changfu

Christou, Paul

Capell Capell, Teresa

Suggested citation

Sanahuja Solsona, Georgina; Farré Martinez, Gemma; Bassie Rene, Ludovic; Zhu, Changfu; Christou, Paul; Capell Capell, Teresa; . (2013) . Ascorbic acid synthesis and metabolism in maize are subject to complex and genotype-dependent feedback regulation duringendosperm development. Biotechnology Journal, 2013, vol. 8, núm. 10, p. 1221-1230. https://doi.org/10.1002/biot.201300064.

Abstract

L-ascorbic acid (vitamin C) is an antioxidant and electron donor whose metabolism in plants isunder strict feedback control. The factors that influence L-ascorbic acid accumulation in staplecrops are only partially understood. One way to gain insight into the regulation of L-ascorbic acidmetabolism is to investigate the endogenous pathways in various genetic backgrounds and char-acterize their interactions with transgenes encoding relevant enzymes. In an initial step, we inves-tigated the developmental profile of L-ascorbic acid accumulation in the endosperm of threediverse maize genotypes and a transgenic line expressing rice dehydroascorbate reductase, whichenhances L-ascorbic acid recycling. We determined the transcript levels of all the key genes in theL-ascorbic acid metabolic pathways as well as the specific levels of ascorbic acid and dehy-droascorbate. L-ascorbic acid levels were high 20 days after pollination and declined thereafter. Wefound significant genotype-dependent variations in the transcript levels of some genes, with par-ticular complexity in the ascorbic acid recycling pathway. Our data will help to elucidate the com-plex mechanisms underlying the regulation of L-ascorbic acid metabolism in plants, particularlythe impact of genetic background on the strict regulation of ascorbic acid metabolism inendosperm cells.

URI

http://hdl.handle.net/10459.1/57160

DOI

https://doi.org/10.1002/biot.201300064

Is part of

Biotechnology Journal, 2013, vol. 8, núm. 10, p. 1221-1230