Ascorbic acid synthesis and metabolism in maize are subject to complex and genotype-dependent feedback regulation duringendosperm development
Sanahuja Solsona, Georgina
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L-ascorbic acid (vitamin C) is an antioxidant and electron donor whose metabolism in plants isunder strict feedback control. The factors that influence L-ascorbic acid accumulation in staplecrops are only partially understood. One way to gain insight into the regulation of L-ascorbic acidmetabolism is to investigate the endogenous pathways in various genetic backgrounds and char-acterize their interactions with transgenes encoding relevant enzymes. In an initial step, we inves-tigated the developmental profile of L-ascorbic acid accumulation in the endosperm of threediverse maize genotypes and a transgenic line expressing rice dehydroascorbate reductase, whichenhances L-ascorbic acid recycling. We determined the transcript levels of all the key genes in theL-ascorbic acid metabolic pathways as well as the specific levels of ascorbic acid and dehy-droascorbate. L-ascorbic acid levels were high 20 days after pollination and declined thereafter. Wefound significant genotype-dependent variations in the transcript levels of some genes, with par-ticular complexity in the ascorbic acid recycling pathway. Our data will help to elucidate the com-plex mechanisms underlying the regulation of L-ascorbic acid metabolism in plants, particularlythe impact of genetic background on the strict regulation of ascorbic acid metabolism inendosperm cells.
Is part ofBiotechnology Journal, 2013, vol. 8, núm. 10, p. 1221-1230
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