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dc.contributor.authorSuz, Laura M.
dc.contributor.authorMartín, Laura P.
dc.contributor.authorColinas, C. (Carlos)
dc.date.accessioned2016-05-31T12:34:17Z
dc.date.issued2006
dc.identifier.issn0378-1097
dc.identifier.urihttp://hdl.handle.net/10459.1/57131
dc.description.abstractOur objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was Z11.4 mg of hyphae g 1 of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.ca_ES
dc.language.isoengca_ES
dc.publisherOxford University Pressca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1111/j.1574-6968.2005.00029.xca_ES
dc.relation.ispartofFEMS Microbiology Letters, 2006, vol. 254, núm. 2, p. 251-257ca_ES
dc.rights(c) Federation of European Microbiological Societies, 2006ca_ES
dc.subjectTuber melanosporumca_ES
dc.subjectPCR-RFLPca_ES
dc.subjectSoil myceliumca_ES
dc.subjectTruffle orchardca_ES
dc.titleDetection of Tuber melanosporum DNA in soilca_ES
dc.typearticleca_ES
dc.identifier.idgrec008190
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/j.1574-6968.2005.00029.x
dc.date.embargoEndDate10000-01-01


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