Detection of Tuber melanosporum DNA in soil
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Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was Z11.4 mg of hyphae g 1 of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.
Is part ofFEMS Microbiology Letters, 2006, vol. 254, núm. 2, p. 251-257
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Mycelial abundance and other factors related to o truffle productivity in Tuber melanosporum - Quercus ilex orchards Suz, Laura M.; María P., Martín; Oliach, Daniel; Fischer, Christine; Colinas, C. (Carlos) (Blackwell, 2008)Relative quantification of DNA from Tuber melanosporum mycelia was performed by conventional and real-time PCR in soil from trees in three truffle orchards of different ages to determine: (1) whether burn appearance ...
Can NPK fertilizers enhance seedling growth and mycorrhizal status of Tuber melanosporum-inoculated Quercus ilex seedlings? Suz, Laura M.; Martín, María P.; Fischer, Christine; Bonet Lledos, José Antonio; Colinas, C. (Carlos) (Springer Verlag, 2010)Although successful cultivation of the black truffle (Tuber melanosporum) has inspired the establishment of widespread truffle orchards in agricultural lands throughout the world, there are many unknowns involved in ...
Fischer, Christine; Colinas, C. (Carlos) (1996)