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dc.contributor.authorFarré Martinez, Gemma
dc.contributor.authorRivera Vélez, Sol Maiam
dc.contributor.authorAlves, Rui
dc.contributor.authorVilaprinyo Terré, Ester
dc.contributor.authorSorribas Tello, Albert
dc.contributor.authorCanela i Garayoa, Ramon
dc.contributor.authorNaqvi, Shaista
dc.contributor.authorSandmann, Gerhard
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorZhu, Changfu
dc.contributor.authorChristou, Paul
dc.date.accessioned2016-05-31T12:17:27Z
dc.date.issued2013
dc.identifier.issn0960-7412
dc.identifier.urihttp://hdl.handle.net/10459.1/57129
dc.description.abstractCarotenoids are a diverse group of tetraterpenoid pigments found in plants, fungi, bacteria and some animals. They play vital roles in plants and provide important health benefits to mammals, including humans. We previously reported the creation of a diverse population of transgenic maize plants expressing various carotenogenic gene combinations and exhibiting distinct metabolic phenotypes. Here we performed an in-depth targeted mRNA and metabolomic analysis of the pathway to characterize the specific impact of five carotenogenic transgenes and their interactions with 12 endogenous genes in four transgenic lines representing distinct genotypes and phenotypes. We reconstructed the temporal profile of the carotenoid pathway during endosperm development at the mRNA and metabolic levels (for total and individual carotenoids), and investigated the impact of transgene expression on the endogenous pathway. These studies enabled us to investigate the extent of any interactions between the introduced transgenic and native partial carotenoid pathways during maize endosperm development. Importantly, we developed a theoretical model that explains these interactions, and our results suggest genetic intervention points that may allow the maize endosperm carotenoid pathway to be engineered in a more effective and predictable manner.ca_ES
dc.description.sponsorshipThis study was supported by the Ministerio de Ciencia e Innovación, Spain (BIO2007-61413, BIO2011-22525 and PM2010PKB-00746 CAROMAIZE) and a European Research Council Advanced Grant (BIOFORCE) to P.C. G.F. is the recipient of a PhD fellowship from the Ministerio de Ciencia e Innovacion, Spain (BES-2008-003894). R.A. and A.S. are partially funded through Research Group 2009SGR809 from the Generalitat de Catalunya and through grants BFU2008-0196 to A.S. and BFU2010-17704 to R.A., both from the Ministerio de Ciencia e Innovacion, Spain.ca_ES
dc.language.isoengca_ES
dc.publisherWileyca_ES
dc.relationMIECI/PN2004-2007/BIO2007-61413ca_ES
dc.relationMICINN/PN2008-2011/BIO2011-22525ca_ES
dc.relationMICINN/PN2008-2011/BES-2008-003894ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1111/tpj.12214ca_ES
dc.relation.ispartofPlant Journal, 2013, vol. 75, núm. 3, p. 441-455ca_ES
dc.rights(c) els autors, 2013
dc.rights(c) Wiley, 2013ca_ES
dc.subjectMetabolic engineeringca_ES
dc.subjectZea maysca_ES
dc.subjectCarotenoidsca_ES
dc.subjectKetocarotenoidsca_ES
dc.titleTargeted transcriptomic and metabolic profiling reveals temporal bottlenecks in the maize carotenoid pathway that may be addressed by multigene engineeringca_ES
dc.typearticleca_ES
dc.identifier.idgrec019371
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/tpj.12214
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/232933ca_ES
dc.date.embargoEndDate10000-01-01


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