Targeted transcriptomic and metabolic profiling reveals temporal bottlenecks in the maize carotenoid pathway that may be addressed by multigene engineering
Data de publicació2013
MetadadesMostra el registre d'unitat complet
Carotenoids are a diverse group of tetraterpenoid pigments found in plants, fungi, bacteria and some animals. They play vital roles in plants and provide important health benefits to mammals, including humans. We previously reported the creation of a diverse population of transgenic maize plants expressing various carotenogenic gene combinations and exhibiting distinct metabolic phenotypes. Here we performed an in-depth targeted mRNA and metabolomic analysis of the pathway to characterize the specific impact of five carotenogenic transgenes and their interactions with 12 endogenous genes in four transgenic lines representing distinct genotypes and phenotypes. We reconstructed the temporal profile of the carotenoid pathway during endosperm development at the mRNA and metabolic levels (for total and individual carotenoids), and investigated the impact of transgene expression on the endogenous pathway. These studies enabled us to investigate the extent of any interactions between the introduced transgenic and native partial carotenoid pathways during maize endosperm development. Importantly, we developed a theoretical model that explains these interactions, and our results suggest genetic intervention points that may allow the maize endosperm carotenoid pathway to be engineered in a more effective and predictable manner.
És part dePlant Journal, 2013, vol. 75, núm. 3, p. 441-455
Projectes de recerca europeus
- Articles publicats (Química) 
- Publicacions de projectes finançats per la Unió Europea 
- Articles publicats (Producció Vegetal i Ciència Forestal) 
- Articles publicats (Ciències Mèdiques Bàsiques) 
- Publicacions de projectes de recerca del Plan Nacional 
- Articles publicats (Agrotecnio Center) 
- Articles publicats (DBA Center) 
Mostrant elements relacionats per títol, autor i matèria.
An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation Bai, Chao; Rivera Vélez, Sol Maiam; Medina Piles, Vicente; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2014)We have developed an assay based on rice embryogenic callus for rapid functional characterization of metabolic genes. We validated the assay using a selection of well-characterized genes with known functions in the carotenoid ...
Synergistic metabolism in hybrid corn indicates bottlenecks in the carotenoid pathway and leads to the accumulation of extraordinary levels of the nutritionally important carotenoid zeaxanthin Naqvi, Shaista; Zhu, Changfu; Farré Martinez, Gemma; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul (Wiley, 2011)Lutein and zeaxanthin cannot be synthesized de novo in humans, and althoughlutein is abundant in fruit and vegetables, good dietary sources of zeaxanthin arescarce. Certain corn varieties provide adequate amounts because ...
A novel carotenoid, 4-keto-a-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus Breitenbach, Jürgen; Bai, Chao; Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu; Sandmann, Gerhard (Elsevier, 2014)Rice endosperm is devoid of carotenoids because the initial biosynthetic steps are absent. The early carotenogenesis reactions were constituted through co-transformation of endosperm-derived rice callus with phytoene ...