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dc.contributor.authorYang, Qingjie
dc.contributor.authorYuan, Dawei
dc.contributor.authorShi, Lianxuan
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorBai, Chao
dc.contributor.authorWen, Nuan
dc.contributor.authorLu, Xiaodan
dc.contributor.authorSandmann, Gerhard
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.date.accessioned2016-05-31T11:56:53Z
dc.date.issued2010
dc.identifier.issn0962-8819
dc.identifier.urihttp://hdl.handle.net/10459.1/57128
dc.description.abstractThe accumulation of carotenoids in plants depends critically on the spatiotemporal expression profiles of the genes encoding enzymes in the carotenogenic pathway. We cloned and characterized the Gentiana lutea zeaxanthin epoxidase (GlZEP) promoter to determine its role in the regulation of carotenogenesis, because the native gene is expressed at high levels in petals, which contain abundant chromoplasts. We transformed tomato (Solanum lycopersicum cv. Micro-Tom) plants with the gusA gene encoding the reporter enzyme b-glucuronidase (GUS) under the control of the GlZEP promoter, and investigated the reporter expression profile at the mRNA and protein levels. We detected high levels of gusA expression and GUS activity in chromoplastcontaining flowers and fruits, but minimal levels in immature fruits containing green chloroplasts, in sepals, leaves, stems and roots. GlZEP-gusA expression was strictly associated with fruit development and chromoplast differentiation, suggesting an evolutionarily-conserved link between ZEP and the differentiation of organelles that store carotenoid pigments. The impact of our results on current models for the regulation of carotenogenesis in plants is discussed.ca_ES
dc.description.sponsorshipOur work was supported by grants from the Spanish Ministry of Science and Innovation (MICINN) (BFU2007-61413); European Research Council Advanced Grant (BIOFORCE) to PC; Aciones complementarias, BIO2007-30738-E MICINN, Spain, grants from the National Natural Science Foundation of China (grant nos. 31070269, 30370123, 39970069) and the Programme for Introducing Talents to Universities (B07017).ca_ES
dc.language.isoengca_ES
dc.publisherSpringer Verlagca_ES
dc.relationinfo:eu-repo/grantAgreement/MEC//BFU2007-61413/ES/ca_ES
dc.relationinfo:eu-repo/grantAgreement/MEC//BIO2007-30738-E/ES/NOVEL ANTIGEN DELIVERY FOR SUSTAINED MUCOSAL PROTECTION AGAINST HIV-1/ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1007/s11248-012-9591-5ca_ES
dc.relation.ispartofTransgenic Research, 2012, vol. 21, núm. 5, p. 1043-1056ca_ES
dc.rights(c) Springer Science Business Media B.V., 2012ca_ES
dc.subjectGentiana luteaca_ES
dc.subjectZeaxanthin epoxidaseca_ES
dc.subjectPromoterca_ES
dc.subjectb-Glucuronidaseca_ES
dc.titleFunctional characterization of the Gentiana lutea zeaxanthin epoxidase (GlZEP) promoter in transgenic tomato plantsca_ES
dc.typearticleca_ES
dc.identifier.idgrec017917
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s11248-012-9591-5
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/232933ca_ES
dc.date.embargoEndDate2025-01-01


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