Show simple item record

dc.contributor.authorReverter Branchat, Gemma
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorSorolla Bardají, Maria Alba
dc.contributor.authorTorre Ruiz, M. A. de la
dc.contributor.authorRos Salvador, Joaquim
dc.date.accessioned2016-05-31T07:58:41Z
dc.date.issued2007
dc.identifier.issn1350-0872
dc.identifier.urihttp://hdl.handle.net/10459.1/57122
dc.description.abstractAlcohol dehydrogenase 1 (Adh1)p catalyses the conversion of acetaldehyde to ethanol, regenerating NAD+. In Saccharomyces cerevisiae, Adh1p is oxidatively modified during ageing and, consequently, its activity becomes reduced. To analyse whether maintaining this activity is advantageous for the cell, a yeast strain with an extra copy of the ADH1 gene (2 ADH1) was constructed, and the effects on chronological and replicative ageing were analysed. The strain showed increased survival in stationary phase (chronological ageing) due to induction of antioxidant enzymes such as catalase and superoxide dismutases. In addition, 2 ADH1 cells displayed an increased activity of silent information regulator 2 (Sir2)p, an NAD+-dependent histone deacetylase, due to a higher NAD+/NADH ratio. As a consequence, a 30 % extension in replicative life span was observed. Taken together, these results suggest that the maintenance of enzymes that participate in NAD+/NADH balancing is important to chronological and replicative life-span parameters.ca_ES
dc.description.sponsorshipThis work has been supported by grants BFU2004-00593/BMC from the Ministerio de Educación y Ciencia (Spain) and SGR2005-00677 from the Generalitat de Catalunya. G. R.-B. and M. A. S. are recipients of PhD fellowships from the Ministerio de Educación y Ciencia (Spain).ca_ES
dc.language.isoengca_ES
dc.publisherMicrobiology Societyca_ES
dc.relationMIECI/PN2004-2007/BFU2004-00593/BMC
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1099/mic.0.2007/009340-0ca_ES
dc.relation.ispartofMicrobiology, 2007, vol. 153, núm. 11, p. 3667-3676ca_ES
dc.rights(c) SGM, 2007ca_ES
dc.titleChronological and replicative life-span extension in Saccharomyces cerevisiae by increased dosage of alcohol dehydrogenase 1ca_ES
dc.typearticleca_ES
dc.identifier.idgrec011503
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1099/mic.0.2007/009340-0
dc.date.embargoEndDate10000-01-01


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record