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dc.contributor.authorIgoshin, Oleg A.
dc.contributor.authorAlves, Rui
dc.contributor.authorSavageau, Michael A.
dc.date.accessioned2016-05-19T08:54:33Z
dc.date.issued2008
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/10459.1/57054
dc.description.abstractBacterial two-component systems (TCS) are key signal transduction networks regulating global responses to environmental change. Environmental signals may modulate the phosphorylation state of sensor kinases (SK). The phosphorylated SK trans- fers the phosphate to its cognate response regulator (RR), which causes physiological response to the signal. Frequently, the SK is bifunctional and, when unphosphorylated, it is also capable of dephosphory- lating the RR. The phosphatase activity may also be modulated by environmental signals. Using the EnvZ/ OmpR system as an example, we constructed math- ematical models to examine the steady-state and kinetic properties of the network. Mathematical modelling reveals that the TCS can show bistable behaviour for a given range of parameter values if unphosphorylated SK and RR form a dead-end complex that prevents SK autophosphorylation. Addi- tionally, for bistability to exist the major dephospho- rylation flux of the RR must not depend on the unphosphorylated SK. Structural modelling and pub- lished affinity studies suggest that the unphosphory- lated SK EnvZ and the RR OmpR form a dead-end complex. However, bistability is not possible because the dephosphorylation of OmpR ~ P is mainly done by unphosphorylated EnvZ. The implications of this potential bistability in the design of the EnvZ/OmpR network and other TCS are discussed.ca_ES
dc.language.isoengca_ES
dc.publisherBlackwellca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1111/j.1365-2958.2008.06221.xca_ES
dc.relation.ispartofMolecular Microbiology, 2008, vol. 68, p. 1196-1215ca_ES
dc.rights(c) Blackwell, 2008ca_ES
dc.titleHysteretic and graded responses in bacterial two-component signal transductionca_ES
dc.typearticleca_ES
dc.identifier.idgrec011508
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/j.1365-2958.2008.06221.x
dc.date.embargoEndDate2025-01-01


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