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dc.contributor.authorLi, Aihong
dc.contributor.authorZhou, Jianbiao
dc.contributor.authorZuckerman, David
dc.contributor.authorDalton, Virginia
dc.contributor.authorLyons, Cheryl
dc.contributor.authorSilverman, Lewis B.
dc.contributor.authorSallan, Stephen E.
dc.contributor.authorGribben, John G.
dc.contributor.authorRué i Monné, Montserrat
dc.date.accessioned2016-05-18T08:04:51Z
dc.date.issued2003
dc.identifier.issn0006-4971
dc.identifier.urihttp://hdl.handle.net/10459.1/57043
dc.description.abstractImmunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements provide clonal markers useful for diagnosis and measurement of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL). We analyzed the sequences of Ig and TCR gene rearrangements obtained at presentation and relapse in 41 children with ALL to study clonal stability, which has important implications for monitoring MRD, during the course of the disease. In 42%, all original Ig and/or TCR sequences were conserved. In 24%, one original sequence was preserved but the other lost, and in 14% the original sequences were conserved with new sequences identified at relapse. In 20% only new sequences were found at relapse. Using primers designed from the novel relapse sequences, the relapse clone could be identified as subdominant clones in the diagnostic sample in 8 of 14 patients. Alteration of these clonal gene rearrangements is a common feature in childhood ALL. MRD detection should include multiple gene targets to minimize false-negative samples or include also multicolor flow cytometry. In some cases the leukemic progenitor cell might arise earlier in lineage before DHJH recombination but retain the capacity to further differentiate into cells capable of altering the pattern of Ig and/or TCR rearrangements.ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Society of Hematologyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1182/blood-2003-05-1455ca_ES
dc.relation.ispartofBlood, 2003, vol. 102, núm. 13, p. 4520-4526ca_ES
dc.rights(c) The American Society of Hematology, 2003ca_ES
dc.titleSequence analysis of clonal immunoglobulin and T-cell receptor gene rearrangements in children with acute lymphoblastic leukemia at diagnosis and at relapse: implications for pathogenesis and for the clinical utility of PCR-based methods of minimal residual disease detectionca_ES
dc.typearticleca_ES
dc.identifier.idgrec009166
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1182/blood-2003-05-1455
dc.date.embargoEndDate2025-01-01


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