Cyclin D1 localizes in the cytoplasm of keratinocytes during skin differentiation and regulates cell–matrix adhesion
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2013Author
Fernández Hernández, Rita
Pérez Fusté, Noel
Aguayo Ortiz, Rafael
Egea Navarro, Joaquim
Suggested citation
Fernández Hernández, Rita;
Rafel i Borrell, Marta;
Pérez Fusté, Noel;
Aguayo Ortiz, Rafael;
Casanova i Seuma, Josep M. (Josep Manel);
Egea Navarro, Joaquim;
...
Garí Marsol, Eloi.
(2013)
.
Cyclin D1 localizes in the cytoplasm of keratinocytes during skin differentiation and regulates cell–matrix adhesion.
Cell Cycle, 2013, vol. 12, núm. 15.
https://doi.org/10.4161/cc.25590.
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The function of Cyclin D1 (CycD1) has been widely studied in the cell nucleus as a regulatory subunit of the cyclindependent
kinases Cdk4/6 involved in the control of proliferation and development in mammals. CycD1 has been also
localized in the cytoplasm, where its function nevertheless is poorly characterized. In this work we have observed that
in normal skin as well as in primary cultures of human keratinocytes, cytoplasmic localization of CycD1 correlated with
the degree of differentiation of the keratinocyte. In these conditions, CycD1 co-localized in cytoplasmic foci with exocyst
components (Sec6) and regulators (RalA), and with β1 integrin, suggesting a role for CycD1 in the regulation of keratinocyte
adhesion during differentiation. Consistent with this hypothesis, CycD1 overexpression increased β1 integrin
recycling and drastically reduced the ability of keratinocytes to adhere to the extracellular matrix. We propose that localization
of CycD1 in the cytoplasm during skin differentiation could be related to the changes in detachment ability of
keratinocytes committed to differentiation.