Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Metabolic remodeling in frataxin-deficient yeast is mediated by Cth2 and Adr1

Thumbnail
View/Open
021768 (1.418Mb)
Sol·licita una còpia
Issue date
2013
Author
Moreno Cermeño, Armando J.
Alsina Obiols, David
Cabiscol Català, Elisa
Tamarit Sumalla, Jordi
Ros Salvador, Joaquim
Suggested citation
Moreno Cermeño, Armando J.; Alsina Obiols, David; Cabiscol Català, Elisa; Tamarit Sumalla, Jordi; Ros Salvador, Joaquim; . (2013) . Metabolic remodeling in frataxin-deficient yeast is mediated by Cth2 and Adr1. Biochimica et Biophysica Acta, 2013, vol. 1833, núm. 12, p. 3326-3337. https://doi.org/10.1016/j.bbamcr.2013.09.019.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Frataxin is a mitochondrial protein involved in iron metabolism whose deficiency in humans causes Friedreich ataxia. We performed transcriptomic and proteomic analyses of conditional Yeast Frataxin Homologue (Yfh1) mutants (tetO7-YFH1) to investigate metabolic remodeling upon Yfh1 depletion. These studies revealed that Yfh1 depletion leads to downregulation of many glucose-repressed genes. Most of them were Adr1 targets, a key transcription factor required for growth in non-fermentable carbon sources. Using a GFP-tagged Adr1, we observed that Yfh1 depletion promotes the export of Adr1 from the nucleus to the cytosol without affecting its protein levels. This effect was also observed upon H2O2 treatment, but not by iron overload/starvation, indicating the presence of a regulatory pathway involved in Adr1 export and inactivation upon stress conditions. We also observed that CTH2, a gene involved in the mRNA degradation of several iron-containing enzymes, was induced upon Yfh1 depletion. Accordingly, decreased levels of aconitase and succinate dehydrogenase were observed. Nevertheless, their levels were maintained in a Δcth2 mutant even in the absence of Yfh1. From these results we can conclude that, in addition to altering iron homeostasis, frataxin depletion involves drastic metabolic remodeling governed by Adr1 and Cth2 that finally leads to downregulation of iron–sulfur proteins and other proteins involved in respiratory metabolism.
URI
http://hdl.handle.net/10459.1/57012
DOI
https://doi.org/10.1016/j.bbamcr.2013.09.019
Is part of
Biochimica et Biophysica Acta, 2013, vol. 1833, núm. 12, p. 3326-3337
European research projects
Collections
  • Articles publicats (IRBLleida) [755]
  • Articles publicats (Ciències Mèdiques Bàsiques) [474]
  • Articles publicats (Grup de Recerca en Estrès Cel·lular i Supervivència en Models Eucariotes) [51]

Related items

Showing items related by title, author, creator and subject.

  • Major targets of iron-induced protein oxidative damage in frataxin-deficient yeasts are magnesium-binding proteins 

    Irazusta, Verónica Patricia; Moreno Cermeño, Armando J.; Cabiscol Català, Elisa; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi (Elsevier, 2008)
    Iron accumulation has been associated with several pathological conditions such as Friedreich ataxia. This human disorder is caused by decreased expression of frataxin. Iron-overload triggers oxidative stress, but the ...
  • Nitric oxide prevents Aft1 activation and metabolic remodeling in frataxindeficient yeast 

    Alsina Obiols, David; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi (Elsevier, 2018)
    Yeast frataxin homolog (Yfh1) is the orthologue of human frataxin, a mitochondrial protein whose deficiency causes Friedreich Ataxia. Yfh1 deficiency activates Aft1, a transcription factor governing iron homeostasis in ...
  • Yeast frataxin mutants display decreased superoxide dismutase activity crucial to promote protein oxidative damage 

    Irazusta, Verónica Patricia; Obis Monné, Èlia; Moreno Cermeño, Armando J.; Cabiscol Català, Elisa; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi (Elsevier, 2010)
    Iron overload is involved in several pathological conditions, including Friedreich ataxia, a disease caused by decreased expression of the mitochondrial protein frataxin. In a previous study, we identified 14 proteins ...

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to