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dc.contributor.authorRodríguez Colman, Maria José
dc.contributor.authorSorolla Bardají, Maria Alba
dc.contributor.authorVall-llaura Espinosa, Núria
dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorRos Salvador, Joaquim
dc.contributor.authorCabiscol Català, Elisa
dc.date.accessioned2016-05-10T09:48:57Z
dc.date.issued2013
dc.identifier.issn0006-3002
dc.identifier.urihttp://hdl.handle.net/10459.1/57011
dc.description.abstractWithin Saccharomyces cerevisiae, Hcm1is a member of the forkhead transcription factor family with a role in chromosome organization. Our group recently described its involvement in mitochondrial biogenesis and stress resistance, and reports here that Hcm1 played a role in adaptation to respiratory metabolism when glucose or nitrogen was decreased. Regulation of Hcm1 activity occurs in at least three ways: i) protein quantity, ii) subcellular localization, and iii) transcriptional activity. Transcriptional activity was measured using a reporter gene fused to a promoter that contains a binding site for Hcm1. We also analyzed the levels of several genes whose expression is known to be regulated by Hcm1 levels and the role of the main kinases known to respond to nutrients. Lack of sucrose-nonfermenting (Snf1) kinase increases cytoplasmic localization of Hcm1, whereas Δtor1 cells showed a mild increase in nuclear Hcm1. In vitro experiments showed that Snf1 clearly phosphorylates Hcm1 while Sch9 exerts a milder phosphorylation. Although in vitroTor1 does not directly phosphorylate Hcm1, in vivo rapamycin treatment increases nuclear Hcm1. We conclude that Hcm1 participates in the adaptation of cells from fermentation to respiratory metabolism during nutrient scarcity. According to our hypothesis, when nutrient levels decrease, Snf1 phosphorylates Hcm1. This results in a shift from the cytoplasm to the nucleus and increased transcriptional activity of genes involved in respiration, use of alternative energy sources, NAD synthesis and oxidative stress resistance.ca_ES
dc.description.sponsorshipThis work has been supported by grants BFU2010-17387 and CSD2007-20 Consolider Ingenio 2010 from the Ministerio de Ciencia e Innovación (Spain) and SGR2009-00196 from the Generalitat de Catalunya. M.J. Rodriguez-Colman is a recipient of a Ph.D. fellowship from the Ministerio de Ciencia e Innovación (Spain).ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationMICINN/PN2008-2011/BFU2010-17387
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.bbamcr.2013.02.015ca_ES
dc.relation.ispartofBiochimica et Biophysica Acta, 2013, vol. 1833, núm. 8ca_ES
dc.rights(c) Elsevier, 2013ca_ES
dc.subjectForkhead transcription factorca_ES
dc.subjectHcm1ca_ES
dc.subjectNutrient limitationca_ES
dc.titleThe FOX transcription factor Hcm1 regulates oxidative metabolism in response to early nutrient limitation in yeast. Role of Snf1 and Tor1/Sch9 kinasesca_ES
dc.typearticleca_ES
dc.identifier.idgrec019666
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.bbamcr.2013.02.015
dc.date.embargoEndDate2025-01-01


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