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dc.contributor.authorYuste Mateos, Víctor J. (Víctor José)
dc.contributor.authorBayascas Ramírez, José Ramón
dc.contributor.authorLlecha Cano, Núria
dc.contributor.authorSánchez-López, Isabel
dc.contributor.authorBoix Torras, Jacint
dc.contributor.authorComella i Carnicé, Joan Xavier
dc.date.accessioned2016-05-10T08:08:14Z
dc.date.issued2001
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10459.1/57004
dc.description.abstractCaspase-activated DNase is responsible for the oligonucleosomal DNA degradation during apoptosis. DNA degradation is thought to be important for multicellular organisms to prevent oncogenic transformation or as a mechanism of viral defense. It has been reported that certain cells, including some neuroblastoma cell lines such as IMR-5, enter apoptosis without digesting DNA in such a way. We have analyzed the causes for the absence of DNA laddering in staurosporine-treated IMR-5 cells, and we have found that most of the molecular mechanisms controlling apoptosis are well preserved in this cell line. These include degradation of substrates for caspases, blockade of cell death by antiapoptotic genes such as Bcl-2 or Bcl-XL, or normal levels and adequate activation of caspase-3. Moreover, these cells display normal levels of caspase-activated DNase and its inhibitory protein, inhibitor of caspase-activated DNase, and their cDNA sequences are identical to those reported previously. Nevertheless, IMR-5 cells lose caspase-activated DNase during apoptosis and recover their ability to degrade DNA when human recombinant caspase-activated DNase is overexpressed. Our results lead to the conclusion that caspase-activated DNase is processed during apoptosis of IMR-5 cells, making these cells a good model to study the relevance of this endonuclease in physiological or pathological conditions.ca_ES
dc.description.sponsorshipThis work was supported by Proyectos FEDER Grant 1FD97-0514- 002-01 and Plan Nacional Salud y Farmacia Grant SAF 2000-0164-002-01) from the Spanish Government and grants from Telemarató de TV3 (Edició 1999) and Generalitat de Catalunyaca_ES
dc.language.isoengca_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyca_ES
dc.relationMICYT/PN2000-2003/SAF2000-0164-C02-01
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1074/jbc.M100072200ca_ES
dc.relation.ispartofJournal of Biological Chemistry, 2001, vol. 276, núm. 25, p. 22323-22331ca_ES
dc.rights(c) The American Society for Biochemistry and Molecular Biology, Inc., 2001ca_ES
dc.titleThe Absence of Oligonucleosomal DNA Fragmentation during Apoptosis of IMR-5 Neuroblastoma Cellsca_ES
dc.typearticleca_ES
dc.identifier.idgrec004930
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1074/jbc.M100072200
dc.date.embargoEndDate10000-01-01


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