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The Absence of Oligonucleosomal DNA Fragmentation during Apoptosis of IMR-5 Neuroblastoma Cells

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Issue date
2001
Author
Yuste Mateos, Víctor J. (Víctor José)
Bayascas Ramírez, José Ramón
Llecha Cano, Núria
Sánchez-López, Isabel
Boix Torras, Jacint
Comella i Carnicé, Joan Xavier
Suggested citation
Yuste Mateos, Víctor J. (Víctor José); Bayascas Ramírez, José Ramón; Llecha Cano, Núria; Sánchez-López, Isabel; Boix Torras, Jacint; Comella i Carnicé, Joan Xavier; . (2001) . The Absence of Oligonucleosomal DNA Fragmentation during Apoptosis of IMR-5 Neuroblastoma Cells. Journal of Biological Chemistry, 2001, vol. 276, núm. 25, p. 22323-22331. https://doi.org/10.1074/jbc.M100072200.
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Abstract
Caspase-activated DNase is responsible for the oligonucleosomal DNA degradation during apoptosis. DNA degradation is thought to be important for multicellular organisms to prevent oncogenic transformation or as a mechanism of viral defense. It has been reported that certain cells, including some neuroblastoma cell lines such as IMR-5, enter apoptosis without digesting DNA in such a way. We have analyzed the causes for the absence of DNA laddering in staurosporine-treated IMR-5 cells, and we have found that most of the molecular mechanisms controlling apoptosis are well preserved in this cell line. These include degradation of substrates for caspases, blockade of cell death by antiapoptotic genes such as Bcl-2 or Bcl-XL, or normal levels and adequate activation of caspase-3. Moreover, these cells display normal levels of caspase-activated DNase and its inhibitory protein, inhibitor of caspase-activated DNase, and their cDNA sequences are identical to those reported previously. Nevertheless, IMR-5 cells lose caspase-activated DNase during apoptosis and recover their ability to degrade DNA when human recombinant caspase-activated DNase is overexpressed. Our results lead to the conclusion that caspase-activated DNase is processed during apoptosis of IMR-5 cells, making these cells a good model to study the relevance of this endonuclease in physiological or pathological conditions.
URI
http://hdl.handle.net/10459.1/57004
DOI
https://doi.org/10.1074/jbc.M100072200
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Journal of Biological Chemistry, 2001, vol. 276, núm. 25, p. 22323-22331
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