Analysis of U1snRNP-specific A protein cross-linked complexes
MetadataShow full item record
The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonuclease digestion, suggesting that UsnRNA may play important roles in the spatial organization of A and other proteins. Moreover, the data suggest that A is proximal to, and may have interactions with, UsnRNP-specific proteins C and 70 kDa as well as with UsnRNP-common proteins B, E and G.
Is part ofFebs Letter, 1990, vol. 272, núm (1-2), p. 213-216
European research projects
Showing items related by title, author, creator and subject.
Serum Deprivation and Protein Synthesis Inhibition Induce Two Different Apoptotic Processes in N18 Neuroblastoma Cells Fibla Palazón, Joan; Yuste Mateos, Víctor J. (Víctor José); Piulats, J.M; Llecha Cano, Núria; Comella i Carnicé, Joan Xavier; Boix Torras, Jacint (Elsevier, 1998)N18 are murine neuroblastoma cells that underwent cell death upon serum deprivation or inhibition of protein synthesis by means of cycloheximide (CHX). In both cases, an ultrastructural morphology and an internucleosomal ...
Cell differentiation, caspase inhibition and macromolecular synthesis blockage, but not Bcl-2 or Bcl-XL proteins, protect SH-SY5Y cells from apoptosis triggered by two cdk inhibitory drugs. Ribas i Fortuny, Judit; Boix Torras, Jacint (Elsevier, 2004)Olomoucine and Roscovitine are two ATP-competing compounds described as specific inhibitors of cyclin-dependent kinases (CDK). Both drugs showed to induce apoptosis in SH-SY5Y, a neuroblastoma-derived cell line. In these ...
Garrofé Ochoa, Xènia; Melero Fernández de Mera, Raquel M; Fernández Gómez, Francisco J.; Ribas i Fortuny, Judit; Jordán Bueso, Joaquín; Boix Torras, Jacint (American Association for Cancer Research, 2008)In previous reports we have shown in SH-SY5 cells that Olomoucine and Roscovitine, two inhibitory drugs of Cyclin Dependent Kinases, caused apoptosis independent of the extrinsic pathway. In this experimental paradigm, ...