Metabolic engineering of ketocarotenoid biosynthesis in higher plants
MetadataShow full item record
Ketocarotenoids such as astaxanthin and canthaxanthin have important applications in the nutraceutical, cosmetic, food and feed industries. Astaxanthin is derived from b-carotene by 3-hydroxylation and 4-ketolation at both ionone end groups. These reactions are catalyzed by b-carotene hydroxylase
and b-carotene ketolase, respectively. The hydroxylation reaction is widespread in higher plants, but ketolation is restricted to a few bacteria, fungi, and some unicellular green algae. The recent cloning and characterization of b-carotene ketolase genes in conjunction with the development of effective co-transformation strategies permitting facile co-integration of multiple transgenes in target plants provided essential resources and tools to produce ketocarotenoids in planta by genetic engineering. In this review, we discuss ketocarotenoid biosynthesis in general, and characteristics and functional properties of b-carotene ketolases in particular. We also describe examples of ketocarotenoid engineering in plants and we conclude by discussing strategies to efficiently convert b-carotene to astaxanthin in transgenic plants.
Is part ofArchives of Biochemistry and Biophysics, 2009, vol. 483, p. 182-190
Showing items related by title, author, creator and subject.
Synergistic metabolism in hybrid corn indicates bottlenecks in the carotenoid pathway and leads to the accumulation of extraordinary levels of the nutritionally important carotenoid zeaxanthin Naqvi, Shaista; Zhu, Changfu; Farré Martinez, Gemma; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul (Wiley, 2011)Lutein and zeaxanthin cannot be synthesized de novo in humans, and althoughlutein is abundant in fruit and vegetables, good dietary sources of zeaxanthin arescarce. Certain corn varieties provide adequate amounts because ...
Targeted transcriptomic and metabolic profiling reveals temporal bottlenecks in the maize carotenoid pathway that may be addressed by multigene engineering Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Naqvi, Shaista; Sandmann, Gerhard; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (Wiley, 2013)Carotenoids are a diverse group of tetraterpenoid pigments found in plants, fungi, bacteria and some animals. They play vital roles in plants and provide important health benefits to mammals, including humans. We ...
Farré Martinez, Gemma; Sanahuja Solsona, Georgina; Naqvi, Shaista; Bai, Chao; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (Elsevier, 2010)The carotenoids are a major class of organic pigments produced in plants and microbes. They fulfill many essential physiological and developmental processes in plants, and also have important roles in animal health and ...