Universitat de Lleida
    • English
    • català
    • español
  • español 
    • English
    • català
    • español
  • Iniciar sesión
Repositori Obert UdL
Ver ítem 
  •   Inicio
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • Ver ítem
  •   Inicio
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Identification of the Major Oxidatively Damaged Proteins in Escherichia coli Cells Exposed to Oxidative Stress

Thumbnail
Ver/Abrir
000363.pdf (216.2Kb)
Fecha de publicación
1998
Autor/a
Tamarit Sumalla, Jordi
Cabiscol Català, Elisa
Ros Salvador, Joaquim
Cita recomendada
Tamarit Sumalla, Jordi; Cabiscol Català, Elisa; Ros Salvador, Joaquim; . (1998) . Identification of the Major Oxidatively Damaged Proteins in Escherichia coli Cells Exposed to Oxidative Stress. Journal of Biological Chemistry, 1998, Vol. 273, núm. 5, p. 3027-3032. https://doi.org/10.1074/jbc.273.5.3027.
Impacto


Logo de Web of Science    citaciones en Web of Science

Logo de Scopus    citaciones en Scopus

Logo de Google Académico  Google Académico
Compartir
Exportar a Mendeley
Metadatos
Mostrar el registro completo del ítem
Resumen
In the present study we have analyzed protein oxidation on Escherichia coli when these cells were submitted to different stress conditions such as hydrogen peroxide, superoxide-generating compounds, and iron overloading. Carbonyl groups on oxidized cell proteins were examined by Western blot immunoassay. When anaerobically grown E. coli cells were exposed to hydrogen peroxide stress, alcohol dehydrogenase E, elongation factor G, the heat shock protein DNA K, oligopeptidebinding protein A, enolase, and the outer membrane protein A were identified as the major protein targets. A similar immunostained band pattern was found when cells were shifted from anaerobic to aerobic conditions in the presence of different concentrations of iron; it is relevant to note that oxidation of outer membrane protein C, not observed in peroxide stress conditions, was clearly detected as the concentration of iron was increased in the culture media. The hydrogen peroxide stress performed under aerobic conditions affected the b-subunit of F0F1-ATPase; the rest of the oxidized protein pattern was very similar to that found for anaerobic conditions, with the exception of alcohol dehydrogenase E, a protein not synthesized aerobically. Cells submitted to superoxide stress using menadione showed a more specific pattern in which elongation factor G and the b-subunit of F0F1-ATPase were affected significantly. When paraquat was used, although the degree of oxidative damage was lower, the same two modified proteins were detected, and DNA K was also clearly damaged. Cell viability was affected to different extents depending on the type of stress exerted. The results described in this paper provide data about the in vivo effects of oxidative stress on protein oxidation and give insights into understanding how such modifications can affect cellular functions.
URI
http://hdl.handle.net/10459.1/56765
DOI
https://doi.org/10.1074/jbc.273.5.3027
Es parte de
Journal of Biological Chemistry, 1998, Vol. 273, núm. 5, p. 3027-3032
Proyectos de investigación europeos
Colecciones
  • Articles publicats (Ciències Mèdiques Bàsiques) [547]

Contacto | Sugerencias | Aviso legal
© 2022 BiD. Universitat de Lleida
Metadatos sujetos a 
 

 

Explorar

Todo el repositorioComunidades y coleccionesPor fecha de publicaciónAutoresTítulosMateriasEsta colecciónPor fecha de publicaciónAutoresTítulosMaterias

Estadísticas

Ver Estadísticas de uso

De interés

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contacto | Sugerencias | Aviso legal
© 2022 BiD. Universitat de Lleida
Metadatos sujetos a