Oxidative Damage to Specific Proteins in Replicative and Chronological-aged Saccharomyces cerevisiae

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2004Suggested citation
Reverter Branchat, Gemma;
Cabiscol Català, Elisa;
Tamarit Sumalla, Jordi;
Ros Salvador, Joaquim;
.
(2004)
.
Oxidative Damage to Specific Proteins in Replicative and Chronological-aged Saccharomyces cerevisiae.
Journal of Biological Chemistry, 2004, vol. 279, núm. 30, p. 31983-31989.
https://doi.org/10.1074/jbc.M404849200.
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Show full item recordAbstract
Oxidative modifications of cellular components have
been described as one of the main contributions to aged
phenotype. In Saccharomyces cerevisiae, two distinct
life spans can be considered, replicative and chronological.
The relationship between both aging models is still
not clear despite suggestions that these phenomena
may be related. In this work, we show that replicative
and chronological-aged yeast cells are affected by an
oxidative stress situation demonstrated by increased
protein carbonylation when compared with young
cells. The data on the identification of these oxidatively
modified proteins gives clues to better understand
cellular dysfunction that occurs during aging.
Strikingly, although in both aging models metabolic
differences are important, major targets are almost the
same. Common targets include stress resistance proteins
(Hsp60 and Hsp70) and enzymes involved in glucose
metabolism such as enolase, glyceraldehydes-3-P
dehydrogenase, fructose-1,6-biphosphate aldolase,
pyruvate decarboxylase, and alcohol dehydrogenase.
In both aging models, calorie restriction results in decreased
damage to these proteins. In addition, chronological-aged
cells grown under glucose restriction displayed
lowered levels of lipid peroxidation product
lipofuscin. Intracellular iron concentration is kept almost
unchanged, whereas in non-restricted cells, the
values increase up 4–5 times. The pro-oxidant effects
of such increased iron concentration would account
for the damage observed. Also, calorie-restricted cells
show undamaged catalase, which clearly appears carbonylated
in cells grown at a high glucose concentration.
These results may explain lengthening of the viability
of chronological-aged cells and could have an
important role in replicative life span extension by
calorie restriction.