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dc.contributor.authorEchave Lozano, Pedro
dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorRos Salvador, Joaquim
dc.date.accessioned2016-03-29T09:39:07Z
dc.date.available2016-03-29T09:39:07Z
dc.date.issued2003
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10459.1/56753
dc.description.abstractAlcohol dehydrogenase E (AdhE) is an Fe-enzyme that, under anaerobic conditions, is involved in dissimilation of glucose. The enzyme is also present under aerobic conditions, its amount is about one-third and its activity is only one-tenth of the values observed under anaerobic conditions. Nevertheless, its function in the presence of oxygen remained ignored. The data presented in this paper led us to propose that the enzyme has a protective role against oxidative stress. Our results indicated that cells deleted in adhE gene could not grow aerobically in minimal media, were extremely sensitive to oxidative stress and showed division defects. In addition, compared with wild type, mutant cells displayed increased levels of internal peroxides (even higher than those found in a katG strain) and increased protein carbonyl content. This pleiotropic phenotype disappeared when the adhE gene was reintroduced into the defective strain. The purified enzyme was highly reactive with hydrogen peroxide (with a Ki of 5 M), causing inactivation due to a metal-catalyzed oxidation reaction. It is possible to prevent this reactivity to hydrogen peroxide by zinc, which can replace the iron atom at the catalytic site of AdhE. This can also be achieved by addition of ZnSO4 to cell cultures. In such conditions, addition of hydrogen peroxide resulted in reduced cell viability compared with that obtained without the Zn treatment. We therefore propose that AdhE acts as a H2O2 scavenger in Escherichia coli cells grown under aerobic conditions.ca_ES
dc.description.sponsorshipThis work was funded by Grants BMC2001-0874 from Ministerio de Ciencia y Tecnología (Spain) and 2000 SGR 0042 from Generalitat de Catalunya (Spain).ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyca_ES
dc.relationMICYT/PN2000-2003/BMC2001-0874
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1074/jbc.M304351200ca_ES
dc.relation.ispartofJournal of Biological Chemistry, 2003, vol. 278, núm. 32, p. 30193-30198ca_ES
dc.rights(c) The American Society for Biochemistry and Molecular Biology, 2003ca_ES
dc.titleNovel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia colica_ES
dc.typearticleca_ES
dc.identifier.idgrec000004
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1074/jbc.M304351200


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