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dc.contributor.authorEchave Lozano, Pedro
dc.contributor.authorEsparza-Cerón, M. Angel
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorRos Salvador, Joaquim
dc.contributor.authorMembrillo-Hernández, Jorge
dc.contributor.authorLin, Edmund C. C.
dc.date.accessioned2016-03-29T08:54:34Z
dc.date.available2016-03-29T08:54:34Z
dc.date.issued2002
dc.identifier.issn1091-6490
dc.identifier.urihttp://hdl.handle.net/10459.1/56752
dc.description.abstractThe adhE gene of Escherichia coli encodes a multifunctional ethanol oxidoreductase (AdhE) that catalyzes successive reductions of acetyl-CoA to acetaldehyde and then to ethanol reversibly at the expense of NADH. Mutant JE52, serially selected for acquired and improved ability to grow aerobically on ethanol, synthesized an AdhEA267T/E568K with two amino acid substitutions that sequentially conferred improved catalytic properties and stability. Here we show that the aerobic growth ability on ethanol depends also on protection of the mutant AdhE against metal-catalyzed oxidation by the chaperone DnaK (a member of the Hsp70 family). No DnaK protection of the enzyme is evident during anaerobic growth on glucose. Synthesis of DnaK also protected E. coli from H2O2 killing under conditions when functional AdhE is not required. Our results therefore suggest that, in addition to the known role of protecting cells against heat stress, DnaK also protects numerous kinds of proteins from oxidative damage.ca_ES
dc.language.isoengca_ES
dc.publisherNational Academy of Sciencesca_ES
dc.relation.isformatofReproducció del document publicat a http//dx.doi.org/10.1073pnas.072504199ca_ES
dc.relation.ispartofPNAS, 2002, vol. 99, núm. 7, p. 4626-4631ca_ES
dc.rights(c) National Academy of Sciences, 2002ca_ES
dc.titleDnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia colica_ES
dc.typearticleca_ES
dc.identifier.idgrec000008
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1073/pnas.072504199
dc.date.embargoEndDate2025-01-01


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