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dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorAguilar, Juan
dc.contributor.authorRos Salvador, Joaquim
dc.date.accessioned2016-03-17T10:58:00Z
dc.date.available2016-03-17T10:58:00Z
dc.date.issued1997
dc.identifier.issn0021-9193
dc.identifier.urihttp://hdl.handle.net/10459.1/56729
dc.description.abstractZymomonas mobilis is endowed with two isoenzymes of fermentative alcohol dehydrogenase, a zinc-containing enzyme (ADH I) and an iron-containing enzyme (ADH II). The activity of ADH I remains fully conserved, while ADH II activity decays when anaerobic cultures are shifted to aerobiosis. This differential response depends on the metal present on each isoenzyme, since pure preparations of ADH I are resistant to oxidative inactivation and preparations of zinc-containing ADH II, obtained by incubation of pure ADH II with ZnCl2, showed no modification of the target for oxidative damage (His277-containing peptide). It was consistently found that the activity of the zinc-containing ADH II, once submitted to oxidative treatment, was fully restored when iron was reintroduced into the enzyme structure. These results indicate that zinc bound to these proteins plays an important role in the protection of their active centers against oxidative damage and may have relevant biochemical and physiological consequences in this species.ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Society for Microbiologyca_ES
dc.relation.isformatofReproducció del document publicat a http://www.ncbi.nlm.nih.gov/pmc/articles/PMC178804/pdf/1791102.pdfca_ES
dc.relation.ispartofJournal of Bacteriology, 1997, vol. 179, núm. 4, p. 110-1104ca_ES
dc.rights(c) American Society for Microbiology, 1997ca_ES
dc.titleDifferential Inactivation of Alcohol Dehydrogenase Isoenzymes in Zymomonas mobilis by Oxygenca_ES
dc.typearticleca_ES
dc.identifier.idgrec000525
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.date.embargoEndDate2025-01-01


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