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dc.contributor.authorValls i Bautista, Cristina
dc.contributor.authorBougel, Stéphanie
dc.contributor.authorPiñol Felis, Carme
dc.contributor.authorViñas Salas, Joan
dc.contributor.authorBenhattar, Jean
dc.date.accessioned2016-02-24T09:37:35Z
dc.date.available2016-02-24T09:37:35Z
dc.date.issued2011
dc.identifier.issn1792-1074
dc.identifier.urihttp://hdl.handle.net/10459.1/56644
dc.description.abstractColorectal cancers exhibit a high telomerase activity, usually correlated with the hypermethylation of the promoter of its hTERT catalytic subunit. Although telomerase is not expressed in normal tissue, certain proliferative somatic cells such as intestinal crypt cells have demonstrated telomerase activity. The aim of this study was to determine whether a correlation exists between telomerase activity, levels of hTERT methylation and telomere length in tumoral and normal colorectal tissues. Tumor, transitional and normal tissues were obtained from 11 patients with a colorectal cancer. After bisulfite modification of genomic DNA, hTERT promoter methylation was analyzed by methylation-sensitive single-strand conformation analysis (MS-SSCA). Telomerase activity and telomere length were measured by a fluorescent‑ telomeric repeat amplification protocol assay and by Southern blotting, respectively. A significant increase of hTERT methylation and telomerase activity, and a reduction of the mean telomere length were observed in the tumor tissues compared to the transitional and normal mucosa. In the transitional and normal mucosa, telomerase activity was significantly lower than that in tumor tissues, even with high levels of hTERT methylation. Nevertheless, hTERT promoter methylation was not linearly correlated to telomerase activity. These data indicate that hTERT promoter methylation is a necessary event for hTERT expression, as is telomerase activity. However, methylation is not sufficient for hTERT activation, particularly in normal colorectal cells.ca_ES
dc.language.isoengca_ES
dc.publisherSpandidos Publicationsca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.3892/ol.2011.386ca_ES
dc.relation.ispartofOncology letters, 2011, vol. 2, núm. 6, p.1257-1260ca_ES
dc.rights(c) Spandidos Publications, 2011ca_ES
dc.subjectTelomeraseca_ES
dc.subjecthTERTca_ES
dc.subjectPromoter methylationca_ES
dc.subjectColorectal cancerca_ES
dc.titlehTERT methylation is necessary but not sufficient for telomerase activity in colorectal cellsca_ES
dc.typearticleca_ES
dc.identifier.idgrec019772
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.3892/ol.2011.386
dc.date.embargoEndDate10000-01-01


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