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dc.contributor.authorPuigpinós Roig, Judit
dc.contributor.authorCasas Herranz, Celia
dc.contributor.authorHerrero Perpiñán, Enrique
dc.description.abstractGlutaredoxin 6 (Grx6) of Saccharomyces cerevisiae is an integral thiol oxidoreductase protein of the endoplasmic reticulum/Golgi vesicles. Its absence alters the redox equilibrium of the reticulum lumen toward a more oxidized state, thus compensating the defects in protein folding/secretion and cell growth caused by low levels of the oxidase Ero1. In addition, null mutants in GRX6 display a more intense unfolded protein response than wild-type cells upon treatment with inducers of this pathway. These observations support a role of Grx6 in regulating the glutathionylation of thiols of endoplasmic reticulum/Golgi target proteins and consequently the equilibrium between reduced and oxidized glutathione in the lumen of these compartments. A specific function influenced by Grx6 activity is the homeostasis of intracellular calcium. Grx6-deficient mutants have reduced levels of calcium in the ER lumen, whereas accumulation occurs at the cytosol from extracellular sources. This results in permanent activation of the calcineurin-dependent pathway in these cells. Some but not all the phenotypes of the mutant are coincident with those of mutants deficient in intracellular calcium transporters, such as the Golgi Pmr1 protein. The results presented in this study provide evidence for redox regulation of calcium homeostasis in yeast cells.ca_ES
dc.description.sponsorshipThis work was supported by Grants BFU2010-17656 (Ministerio de Economía y Competitividad, Spain) and 2009/SGR/196 (Generalitat de Catalunya).ca_ES
dc.publisherAmerican Society for Cell Biologyca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofMolecular Biology of the Cell, 2015, vol. 26, núm. 1, p. 104-116ca_ES
dc.rightscc-by-nc-sa (c) Puigpinós et al., 2015ca_ES
dc.titleAltered intracellular calcium homeostasis and endoplasmic reticulum redox state in Saccharomyces cerevisiae cells lacking Grx6 glutaredoxinca_ES

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cc-by-nc-sa (c) Puigpinós et al., 2015
Except where otherwise noted, this item's license is described as cc-by-nc-sa (c) Puigpinós et al., 2015