The physical form of corn influences the rumen bacterial biodiversity-preliminary results
Newbold, C. Jamie
MetadataShow full item record
The aim of this study was to investigate the rumen bacteria in terms of genetic biodiversity and variation due to different physical form of corn in cow diet. A total of twenty dry cows were fed for 3 months with the same diet, only differed for corn physical form, ten received corn grains, while the
other ones received corn flour. To investigate the biodiversity of the bacterial 16S rRNA gene clone library analysis has been conducted and then the sequencing has been carried out using Ion Torrent PGM¿ System. Bacterial population was tested using R statistical software. The Kruskal-Wallis one-way analysis of variance (Kruskal-Wallis, 1952) confirmed that the bacterial populations were different when the animals were fed grain compared with flour corn. Both the OUT's abundance (Operational Taxonomic Unit) and the biodiversity indexes presented a significant difference among the two sample groups, underlining the large changes that take place even with small diet modifications in ruminal environment. There is still the need to deepen how exactly the diet changes the rumen phylogenetic structure and the consequences on bacteria's activity.
Is part ofPoljoprivreda, 2015, vol. 21, núm. 1, suplement, p. 93-96
Showing items related by title, author, creator and subject.
Pros and cons of ion-torrent next generation sequencing versus terminal restriction fragment length polymorphism T-RFLP for studying the rumen bacterial community de la Fuente Oliver, Gabriel; Belanche, A.; Girwood, Susan E.; Pinloche, Eric; Wilkinson, Toby; Newbold, C. Jamie (Public Library of Science, 2014-07-22)The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following ...
Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis Belanche, A.; de la Fuente Oliver, Gabriel; Pinloche, Eric; Newbold, C. Jamie; Balcells Terés, Joaquim (American Society of Animal Science, 2012-04-21)Accurate estimates of microbial synthesis in the rumen are vital to optimize ruminant nutrition. Liquid- (LAB) and solid-associated bacterial fractions (SAB) harvested from the rumen are generally considered as microbial ...
Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Raymond J. Geor; Nielsen, Brian D.; Schott II, Harold C.; Elzinga, Sarah; Newbold, C. Jamie (Public Library of Science, 2014-02-04)Faecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1-V2 regions ...