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dc.contributor.authorBelanche, A.
dc.contributor.authorde la Fuente Oliver, Gabriel
dc.contributor.authorPinloche, Eric
dc.contributor.authorNewbold, C. Jamie
dc.contributor.authorBalcells Terés, Joaquim
dc.date.accessioned2015-12-22T16:55:33Z
dc.date.available2015-12-22T16:55:33Z
dc.date.issued2012-04-21
dc.identifier.issn0021-8812
dc.identifier.urihttp://hdl.handle.net/10459.1/49285
dc.description.abstractAccurate estimates of microbial synthesis in the rumen are vital to optimize ruminant nutrition. Liquid- (LAB) and solid-associated bacterial fractions (SAB) harvested from the rumen are generally considered as microbial references when microbial yield is calculated; however, factors that determine their composition are not completely understood. The aim of this study was to evaluate the effect of diet and absence or presence of rumen protozoa on the rumen microbial community. It was hypothesized that these treatments could modify the composition and representativeness of LAB and SAB. Twenty twin lambs (Ovis aries) were used; one-half of the twins were kept protozoa-free, and each respective twin sibling was faunated. At 6 mo of age, 5 animals from each group were randomly allocated to the experimental diets consisting of either alfalfa hay as the sole diet, or 50:50 mixed with ground barley grain. After 15 d of adaptation to the diet, animals were euthanized, rumen and abomasum contents were sampled, and LAB and SAB isolated. The presence of protozoa buffered the effect of diet on the rumen bacterial population. Faunated animals fed alfalfa hay had a greater abundance of F. succinogenes, anaerobic fungi and methanogens, as well as an enhanced rumen bacterial diversity. Cellulolytic bacteria were more abundant in SAB, whereas the abomasal abundance of most of the microorganisms studied was closer to those values observed in LAB. Rumen and abomasal samples showed similar bacterial DNA concentrations, but the fungal and protozoal DNA concentration in the abomasum was only 69% and 13% of that observed in the rumen, respectively, suggesting fungal and protozoal sequestration in the rumen or possible preferential degradation of fungal and protozoal DNA in the abomasum, or both. In conclusion, absence of protozoa and type of diet extensively modifi ed the chemical composition of LAB and SAB as a consequence of changes in the microbial composition of these fractions.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Society of Animal Science
dc.relation.isformatofReproducció del document publicat a: http://dx.doi.org/10.2527/jas.2011-4802
dc.relation.ispartofJournal of Animal Science, 2012, vol. 90, num. 11, p. 3924-3936
dc.rights(c) American Society of Animal Science, 2012
dc.subject.classificationRemugants
dc.subject.classificationSíntesi proteica
dc.subject.otherRuminants
dc.subject.otherProtein synthesis
dc.titleEffect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-12-15T18:37:32Z
dc.identifier.idgrec023071
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.2527/jas.2011-4802


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