Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
Valls i Bautista, Cristina
Buenestado Garcia, Juan
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Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenomacarcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps.
Is part ofRevista Española de Enfermedades Digestivas, 2009, vol. 101, núm. 3
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Valls i Bautista, Cristina; Piñol Felis, Carme; Reñé Espinet, Josep Maria; Buenestado Garcia, Juan; Viñas Salas, Joan (Wiley, 2011)Aim The aim of this study was to determine whether telomere length is an independent prognostic factor for the prevention and survival of colorectal cancer. Method Terminal restriction fragment (TRF) length was determined ...
Valls i Bautista, Cristina; Piñol Felis, Carme; Reñé Espinet, Josep Maria; Buenestado Garcia, Juan; Viñas Salas, Joan (Sociedad Española de Patología DigestivaArán Ediciones, 2012)Background: colorectal cancer is the third cancer cause of death in Spain. It is important to investigate new tumoral markers for early diagnosis, disease monitoring and prevention strategies. Telomeres protect the ...
Valls i Bautista, Cristina; Piñol Felis, Carme; Reñé Espinet, Josep Maria; Buenestado Garcia, Juan; Viñas Salas, Joan (Wiley Online Library, 2015-04-17)Background: Telomere length (TL) shortened occurs in colorectal carcinogenetic process. Our objective is to determine if it is only a local fact or there are alterations in normal colon cells and in other body cells. ...