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dc.contributor.authorBelanche, A.
dc.contributor.authorde la Fuente Oliver, Gabriel
dc.contributor.authorNewbold, C. Jamie
dc.date.accessioned2015-12-02T18:39:37Z
dc.date.available2015-12-02T18:39:37Z
dc.date.issued2015-01-08
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10459.1/49223
dc.description.abstractProtozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7<br>3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67% similarity within treatment) in the rumen with similar diversity but different structures than PAM (P< 0.05). On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P< 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.
dc.description.sponsorshipThis work was supported by the Commission of the European Communities (REDNEX project FP7-KBBE-2007-1) and the Welsh Government.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1111/1574-6941.12423
dc.relation.ispartofFems Microbiology Ecology, 2015, vol. 90, núm. 3, p. 663-677
dc.rightscc-by-nc, (c) Belanche, A. et al., 2015
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0
dc.subjectarchaea
dc.subjectendosymbiotic
dc.subjectholotrich
dc.subjectmethanogens
dc.subjectrumen protozoa
dc.subject.classificationRemugants
dc.subject.classificationProtozous
dc.subject.otherRuminants
dc.subject.otherProtozoa
dc.titleStudy of methanogen communities associated with different rumen protozoal populations
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-12-02T18:39:37Z
dc.identifier.idgrec023063
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1111/1574-6941.12423
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/211606


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cc-by-nc, (c) Belanche, A. et al., 2015
Except where otherwise noted, this item's license is described as cc-by-nc, (c) Belanche, A. et al., 2015