Manual Rupture Versus Transvaginal Ultrasound-guided Aspiration of Allanto-amniotic Fluid in Multiple Pregnancies: A Clinical Approach to Embryo Reduction in Dairy Cattle
MetadataShow full item record
To avoid the problems associated with twinning in dairy cattle, one of the embryos may be eliminated. This study compares the effect on pregnancy maintenance of two embryo reduction techniques, manual rupture (MR) and transvaginal ultrasound-guided aspiration (TUGA) of allanto-amniotic fluid, in Holstein-Friesian cows with multiple pregnancies. In the first experiment, 61 lactating cows bearing unilateral twins (n=27), bilateral twins (n=30) or triplets/quadruplets (n=4) were subjected to MR (n=45) or TUGA using a 17-G neddle (n=16) on day 28-34 of gestation. In 21 and 10 cows undergoing MR and TUGA embryo reduction, respectively, pregnancy loss occurred before day 90 (46.7 vs. 62.5%, P= 0.28). Through binary logistic regression, the type of pregnancy was identified as the only variable significantly affecting pregnancy maintenance (P=0.03). Based on the odds ratio, the risk of pregnancy loss was 4.1 times higher for unilateral twins than for bilateral twins (70.4 vs. 36.7%, respectively, P=0.01). No effect was detected on pregnancy maintenance of the technique used (P=0.17) or of the interaction technique by type of pregnancy (P=0.22). In the second experiment, a 22-G needle was used to perform TUGA on 22 lactating cows. The pregnancy loss rates were 44.4% (4/9), 18.2% (2/11) and 50% (1/2) for cows bearing unilateral twins, bilateral twins and triplets, respectively. The total pregnancy loss rate following TUGA using the 22-G needle tended to be lower than that using the 17-G needle (31.8 vs. 62.5%; P=0.06). Our results suggest that TUGA using a 22-G needle could be the method of choice to perform embryo reduction in cows carrying multiple pregnancies.
Is part ofJournal of Reproduction and Development, 2012, vol. 58, núm. 4, p. 420-424
European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Andreu Vazquez, Cristina et al., 2012
Showing items related by title, author, creator and subject.
Transfer of a single embryo versus drainage of subordinate follicles to prevent twin pregnancies in dairy cows. Why not both? López Gatius, Fernando; García Ispierto, Irina (Society for Reproduction and Development, 2020-04-07)In this study, we present two proposed approaches to prevent twin pregnancies in dairy cattle: 1) single, in vitro-produced embryonic transfer into a recipient cow or 2) subordinate follicle drainage at the time of ...
Relationship between heat stress during the peri-implantation period and early fetal loss in dairy cattle García Ispierto, Irina; López Gatius, Fernando; Santolaria Blasco, Pilar; Yániz Pérez de Albéniz, Jesús; Nogareda, Carmina; López-Béjar, M.; De Rensis, F. (Elsevier, 2006)The aim of the present study was to establish whether temperature-humidity index values, as a measure of heat comfort, from Days 1 to 40 of gestation could be associated with the pregnancy loss rate in high producing dairy ...
To what extent does photoperiod affect cattle reproduction? Clinical perspectives of melatonin administration De Rensis, F.; Morini, Giorgio; García Ispierto, Irina; López Gatius, Fernando (De Gruyter Open, 2020-08-01)The seasonality of reproduction in most mammals is dictated by photoperiod, temperature and nutrition. Melatonin, mainly synthesized in the pineal gland, is generally accepted as the active mediator of photoperiod responses ...