Differential interactions of virulent and non-virulent H. parasuis strains with naive or swine influenza virus pre-infected dendritic cells
MetadataShow full item record
Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms
involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.
Is part ofVeterinary Research, 2012, vol. 43, núm 80
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Mussá, Tufária et al., 2012
Showing items related by title, author, creator and subject.
Solórzano, Alicia; Foni, Emanuela; Córdoba, Lorena; Baratelli, Massimiliano; Razzuoli, Elisabetta; Bilato, Dania; Martín del Burgo, María Ángeles; Perlin, David S.; Martínez, Jorge; Martínez-Orellana, Pamela; Fraile Sauce, Lorenzo José; Chiapponi, Chiara; Amadori, Massimo; Del Real, Gustavo; Montoya, Maria (American Society for Microbiology, 2015)Avian influenza A viruses have gained increasing attention due to their ability to cross the species barrier and cause severe disease in humans and other mammal species as pigs. H3 and particularly H3N8 viruses, are highly ...
Differential viral-host immune interactions associated with Oseltamivir-resistant H275Y and wild type H1N1 A(pdm09) Influenza virus pathogenicity Vidaña, Beatriz; Martinez-Orellana, Pamela; Martorell, Jaume; Baratelli, Massimiliano; Martínez, Jorge; García-Migura, Lourdes; Cordoba, Lorena; Pérez, Mónica; Casas, Inmaculada; Pozo, Francisco; Fraile Sauce, Lorenzo José; Majó, Natalia; Montoya, Maria (MDPI, 2020)Oseltamivir is a common therapy against influenza A virus (IAV) infections. The acquisition of oseltamivir resistance (OR) mutations, such as H275Y, hampers viral fitness. However, OR H1N1 viruses have demonstrated the ...
Cabezón Ponsoda, Óscar; Muñoz González, Sara; Colom-Cadena, Andreu; Rosell, Rosa; Lavín, Santiago; Marco, Ignasi; Fraile Sauce, Lorenzo José; Martínez de la Riva, Paloma; Rodríguez, Fernando; Domínguez, Javier; Ganges, Llilianne (BioMed Central, 2017)Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate ...