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dc.contributor.authorBelanche, A.
dc.contributor.authorde la Fuente Oliver, Gabriel
dc.contributor.authorNewbold, C. Jamie
dc.date.accessioned2015-11-25T15:26:58Z
dc.date.issued2014-12-15
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10459.1/49059
dc.description.abstractRumen methanogenesis represents an energy waste for the ruminant and an important source of greenhouse gas; thus, integrated studies are needed to fully understand this process. Eight fauna-free sheep were used to investigate the effect of successive inoculation with holotrich protozoa then with total fauna on rumen methanogenesis. Holotrichs inoculation neither altered rumen fermentation rate nor diet digestibility, but increased concentrations of acetate (+15%), butyrate (+57%), anaerobic fungi (+0.82 log), methanogens (+0.41 log) and methanogenesis (+54%). Further inoculation with total fauna increased rumen concentrations of protozoa (+1.0 log), bacteria (+0.29 log), anaerobic fungi (+0.78 log), VFA (+8%), ammonia and fibre digestibility (+17%) without affecting levels of methanogens or methanogenesis. Rumen methanogens population was fairly stable in terms of structure and diversity, while the bacterial community was highly affected by the treatments. Inoculation with holotrich protozoa increased bacterial diversity. Further inoculation with total fauna lowered bacterial diversity but increased concentrations of certain propionate and lactate-producing bacteria, suggesting that alternative H2 sinks could be relevant. This experiment suggests that holotrich protozoa have a greater impact on rumen methanogenesis than entodiniomorphids. Thus, further research is warranted to understand the effect of holotrich protozoa on methane formation and evaluate their elimination from the rumen as a potential methane mitigation strategy.
dc.description.sponsorshipThis work was supported by the Commission of the European Communities (REDNEX project FP7-KBBE-2007-1) and the Welsh Government
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1093/femsec/fiu026
dc.relation.ispartofFems Microbiology Ecology, 2014, vol. 91, núm. 3
dc.rightscc-by-nc, (c) Fems Microbiology Ecology , 2014
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0
dc.subjectmethanogens
dc.subjectholotrich
dc.subjectrumen protozoa
dc.subject.classificationBestiar oví
dc.subject.classificationRemugants
dc.subject.classificationProtozous
dc.subject.classificationExcrements
dc.subject.otherSheep
dc.subject.otherRuminants
dc.subject.otherProtozoa
dc.subject.otherFeces
dc.titleEffect of progressive inoculation of fauna-free sheep with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-11-25T15:26:58Z
dc.identifier.idgrec023064
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1093/femsec/fiu026
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/211606


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cc-by-nc, (c) Fems Microbiology Ecology , 2014
Except where otherwise noted, this item's license is described as cc-by-nc, (c) Fems Microbiology Ecology , 2014