Identification of a core bacterial community within the large intestine of the horse
Harris, Patricia A.
Girdwood, Susan E.
Newbold, C. Jamie
MetadataShow full item record
The horse has a rich and complex microbial community within its gastrointestinal tract that plays a central role in both health and disease. The horse receives much of its dietary energy through microbial hydrolysis and fermentation of fiber predominantly in the large intestine/hindgut. The presence
of a possible core bacterial community in the equine large intestine was investigated in this study. Samples were taken from the terminal ileum and 7 regions of the large intestine from ten animals, DNA extracted and the V1-V2 regions of 16SrDNA 454-pyrosequenced. A specific group of OTUs clustered in all ileal samples and a distinct and different signature existed for the proximal regions of the large intestine and the distal regions. A core group of bacterial families were identified in all gut regions with clear differences shown between the ileum and the various large intestine regions. The core in the ileum accounted for 32% of all sequences and comprised of only seven OTUs of varying abundance; the core in the large intestine was much smaller (5-15% of all sequences) with a much larger number of OTUs present but in low abundance. The most abundant member of the core community in the ileum was Lactobacillaceae, in the proximal large intestine the Lachnospiraceae and in the distal large intestine the Prevotellaceae. In conclusion, the presence of a core bacterial community in the large intestine of the horse that is made up of many low abundance OTUs may explain in part the susceptibility of horses to digestive upset.
Is part ofPlos One, 2013, vol. 8, núm. 10
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Dougal, Kirsty et al., 2013
Showing items related by title, author, creator and subject.
Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Raymond J. Geor; Nielsen, Brian D.; Schott II, Harold C.; Elzinga, Sarah; Newbold, C. Jamie (Public Library of Science, 2014-02-04)Faecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1-V2 regions ...
Pros and cons of ion-torrent next generation sequencing versus terminal restriction fragment length polymorphism T-RFLP for studying the rumen bacterial community de la Fuente Oliver, Gabriel; Belanche, A.; Girwood, Susan E.; Pinloche, Eric; Wilkinson, Toby; Newbold, C. Jamie (Public Library of Science, 2014-07-22)The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following ...
Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis Belanche, A.; de la Fuente Oliver, Gabriel; Pinloche, Eric; Newbold, C. Jamie; Balcells Terés, Joaquim (American Society of Animal Science, 2012-04-21)Accurate estimates of microbial synthesis in the rumen are vital to optimize ruminant nutrition. Liquid- (LAB) and solid-associated bacterial fractions (SAB) harvested from the rumen are generally considered as microbial ...