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dc.contributor.authorGutierrez, Humberto
dc.contributor.authorDolcet Roca, Xavier
dc.contributor.authorTolcos, Mary
dc.contributor.authorDavies, Alun M.
dc.date.accessioned2015-11-19T14:02:55Z
dc.date.available2015-11-19T14:02:55Z
dc.date.issued2004-08
dc.identifier.issn0950-1991
dc.identifier.urihttp://hdl.handle.net/10459.1/49011
dc.description.abstractAlthough hepatocyte growth factor (HGF) and its receptor tyrosine kinase MET are widely expressed in the developing and mature central nervous system, little is known about the role of MET signaling in the brain. We have used particle-mediated gene transfer in cortical organotypic slice cultures established from early postnatal mice to study the effects of HGF on the development of dendritic arbors of pyramidal neurons. Compared with untreated control cultures, exogenous HGF promoted a highly significant increase in dendritic growth and branching of layer 2 pyramidal neurons, whereas inactivation of endogenous HGF with function-blocking, anti-HGF antibody caused a marked reduction in size and complexity of the dendritic arbors of these neurons. Furthermore, pyramidal neurons transfected with an MET dominant-negative mutant receptor likewise had much smaller and less complex dendritic arbors than did control transfected neurons. Our results indicate that HGF plays a role in regulating dendritic morphology in the developing cerebral cortex.ca_ES
dc.language.isoengca_ES
dc.publisherCompany of Biologists Ltd.ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1242/dev.01209ca_ES
dc.relation.ispartofDevelopment, 2004, vol. 131, núm.15, p. 3717-3726ca_ES
dc.rights(c) Company of Biologists Ltd., 2004ca_ES
dc.subjectMETca_ES
dc.subjectNeuronsca_ES
dc.subjectProcess growthca_ES
dc.subjectSlice culturesca_ES
dc.subject.otherNeuronesca_ES
dc.titleHGF regulates the development of cortical pyramidal dendritesca_ES
dc.typearticleca_ES
dc.identifier.idgrec021106
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1242/dev.01209


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