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dc.contributor.authorDougal, Kirsty
dc.contributor.authorde la Fuente Oliver, Gabriel
dc.contributor.authorHarris, Patricia A.
dc.contributor.authorGirdwood, Susan E.
dc.contributor.authorPinloche, Eric
dc.contributor.authorRaymond J. Geor
dc.contributor.authorNielsen, Brian D.
dc.contributor.authorSchott II, Harold C.
dc.contributor.authorElzinga, Sarah
dc.contributor.authorNewbold, C. Jamie
dc.date.accessioned2015-11-16T19:12:35Z
dc.date.available2015-11-16T19:12:35Z
dc.date.issued2014-02-04
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10459.1/48969
dc.description.abstractFaecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1-V2 regions of 16SrDNA were 454-pyrosequenced to investigate the faecal microbiome of the horse. The effect of age was also considered by comparing mature (8 horses aged 5-12) versus elderly horses (9 horses aged 19-28). A reduction in diversity was found in the elderly horse group. Significant differences between diets were found at an OTU level (52 OTUs at corrected Q<0.1). The majority of differences found were related to the Firmucutes phylum (37) with some changes in Bacteroidetes (6), Proteobacteria (3), Actinobacteria (2) and Spirochaetes (1). For the forage only diet,with no added starch or oil, we found 30/2934 OTUs (accounting for 15.9% of sequences) present in all horses. However the core (i.e. present in all horses) associated with the oil rich supplemented diet was somewhat smaller (25/3029 OTUs, 10.3% ) and the core associated with the starch rich supplemented diet was even smaller (15/2884 OTUs, 5.4% ). The core associated with samples across all three diets was extremely small (6/5689 OTUs accounting for only 2.3% of sequences) and dominated by the order Clostridiales, with the most abundant family being Lachnospiraceae. In conclusion, forage based diets plus starch or oil rich complementary feeds were associated with differences in the faecal bacterial community compared with the forage alone. Further, as observed in people, ageing is associated with a reduction in bacterial diversity. However there was no change in the bacterial community structure in these healthy animals associated with age.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0087424
dc.relation.ispartofPlos One, 2014, vol. 9, núm. 2, e0087424
dc.rightscc-by (c) Dougal, Kirsty et al., 2014
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es
dc.subject.classificationCavalls
dc.subject.classificationExcrements
dc.subject.classificationNutrició animal
dc.subject.classificationBacteris
dc.subject.classificationFibra alimentària
dc.subject.classificationMidó
dc.subject.otherHorses
dc.subject.otherFeces
dc.subject.otherAnimal nutrition
dc.subject.otherBacteria
dc.subject.otherFiber in human nutrition
dc.subject.otherStarch
dc.titleCharacterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-11-16T19:12:36Z
dc.identifier.idgrec023066
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0087424


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cc-by (c) Dougal, Kirsty et al., 2014
Except where otherwise noted, this item's license is described as cc-by (c) Dougal, Kirsty et al., 2014