Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing
Harris, Patricia A.
Girdwood, Susan E.
Raymond J. Geor
Nielsen, Brian D.
Schott II, Harold C.
Newbold, C. Jamie
MetadataShow full item record
Faecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1-V2 regions of 16SrDNA were 454-pyrosequenced to investigate the faecal microbiome of the horse. The effect of age was also considered by comparing mature (8 horses aged 5-12) versus elderly horses (9 horses aged 19-28). A reduction in diversity was found in the elderly horse group. Significant differences between diets were found at an OTU level (52 OTUs at corrected Q<0.1). The majority of differences found were related to the Firmucutes phylum (37) with some changes in Bacteroidetes (6), Proteobacteria (3), Actinobacteria (2) and Spirochaetes (1). For the forage only diet,with no added starch or oil, we found 30/2934 OTUs (accounting for 15.9% of sequences) present in all horses. However the core (i.e. present in all horses) associated with the oil rich supplemented diet was somewhat smaller (25/3029 OTUs, 10.3% ) and the core associated with the starch rich supplemented diet was even smaller (15/2884 OTUs, 5.4% ). The core associated with samples across all three diets was extremely small (6/5689 OTUs accounting for only 2.3% of sequences) and dominated by the order Clostridiales, with the most abundant family being Lachnospiraceae. In conclusion, forage based diets plus starch or oil rich complementary feeds were associated with differences in the faecal bacterial community compared with the forage alone. Further, as observed in people, ageing is associated with a reduction in bacterial diversity. However there was no change in the bacterial community structure in these healthy animals associated with age.
Is part ofPlos One, 2014, vol. 9, núm. 2, e0087424
European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Dougal, Kirsty et al., 2014
Showing items related by title, author, creator and subject.
Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Newbold, C. Jamie (Public Library of Science, 2013-10-24)The horse has a rich and complex microbial community within its gastrointestinal tract that plays a central role in both health and disease. The horse receives much of its dietary energy through microbial hydrolysis and ...
Pros and cons of ion-torrent next generation sequencing versus terminal restriction fragment length polymorphism T-RFLP for studying the rumen bacterial community de la Fuente Oliver, Gabriel; Belanche, A.; Girwood, Susan E.; Pinloche, Eric; Wilkinson, Toby; Newbold, C. Jamie (Public Library of Science, 2014-07-22)The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following ...
Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis Belanche, A.; de la Fuente Oliver, Gabriel; Pinloche, Eric; Newbold, C. Jamie; Balcells Terés, Joaquim (American Society of Animal Science, 2012-04-21)Accurate estimates of microbial synthesis in the rumen are vital to optimize ruminant nutrition. Liquid- (LAB) and solid-associated bacterial fractions (SAB) harvested from the rumen are generally considered as microbial ...